| Anorectal neural crest derived cell behavior after the migration of vagal neural crest derived cells is surgically disrupted: implications for the etiology of Hirschsprung's disease. | |
| | |
MedLine Citation:
|
PMID: 23143079 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
BACKGROUND/PURPOSE: In Hirschsprung's disease (HD), thick extrinsic nerve fibers can be associated with the aganglionic segment in the anorectum. We surgically disrupted the migration of vagal neural crest cell-derived cells (vagal NCC) in embryos from transgenic mice we created previously (SOX10-VENUS Tg) which have the SOX10 gene labeled with Venus (V), a green fluorescent protein, to observe sacral NCC activity in the anorectum. METHOD: Proximal colon harvested from SOX10-VENUS Tg embryos on day 10.5 (n = 10) was transected at the ascending colon. V-positive sacral NCC in the anorectum were observed during organ culture under fluorescence stereoscopic microscopy, and compared with non-transected control specimens (n = 10). RESULTS: In transected specimens, no V-positive sacral NCC were identified initially in the anorectum. By day 2, there were thick beaded sacral NCC in the anorectum in 6/10 (60 %) that migrated steadily to the transected end over 3-4 days. In controls, thinner and shorter V-positive sacral NCC began migrating cranially on day 2, and were met by distally migrating vagal NCC. CONCLUSION: Disruption of vagal NCC migration appears to induce sacral NCC activity in the anorectum, suggesting that thick extrinsic nerve fibers seen in HD may be a secondary phenomenon. |
| | |
Authors:
|
Katsumi Miyahara; Yoshifumi Kato; Ryota Suzuki; Chihiro Akazawa; Nana Tanaka; Hiroyuki Koga; Takashi Doi; Geoffrey J Lane; Atsuyuki Yamataka |
Related Documents
:
|
23124969 - The discontinuous nature of neurofilament transport accommodates both establishment and... 3415989 - Kinetics of enzyme-mediated reduction of lipid soluble nitroxide spin labels by living ... 7669509 - Red blood cell magnesium concentrations: analytical problems and significance. 134119 - Early steps in specific tumor cell lysis by sensitized mouse t lymphocytes. ii. electro... 6267769 - Intracytoplasmic granules of the inner medulla and papilla of the potassium depleted hu... 23437169 - Dishevelled proteins are associated with olfactory sensory neuron presynaptic terminals. 22532929 - Generation of retinal pigment epithelial cells from small molecules and oct4-reprogramm... 946719 - Electrophysiological and anatomical effects of cetylpyridinium chloride on the rabbit c... 1021599 - Aspartate aminotransferase activity and glutamic dehydrogenase in the cerebellar cortex... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-11-11 |
Journal Detail:
|
Title: Pediatric surgery international Volume: - ISSN: 1437-9813 ISO Abbreviation: Pediatr. Surg. Int. Publication Date: 2012 Nov |
Date Detail:
|
Created Date: 2012-11-12 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8609169 Medline TA: Pediatr Surg Int Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Pediatric General and Urogenital Surgery, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Abnormal control of lung branching in experimental esophageal atresia.
Next Document: The Impact of Training Interventions on Organizational Readiness to Support Innovations in Juvenile ...