| Anomalously high concentrations of brain extracellular uric acid detected with chronically implanted probes: implications for in vivo sampling techniques. | |
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MedLine Citation:
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PMID: 2051164 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The height of peak 2, h2, recorded using linear sweep voltammetry with 350-micron-diameter carbon paste electrodes in rat striatum was measured from the day of implantation (day 0) to 4 months after surgery. The value of h2 was at a minimum on day 0 (0.6 +/- 0.2 nA; n = 20), rose sharply to a maximum on day 2 (6.3 +/- 0.9 nA; n = 12), and decreased to a stable level by day 7 (3.3 +/- 0.7 nA; n = 16), which lasted for 4 months (3.2 +/- 0.6 nA; n = 9). These changes were shown by microinfusion of uricase to be due to variations in the concentrations of extracellular uric acid, although h2 appears to have a small baseline contribution of approximately 0.3 nA from 5-hydroxyindoleacetic acid. The stable value of h2 recorded under chronic conditions was estimated to correspond to a minimal uric acid concentration of 50 mumol/L, which represents a 10-fold increase in the extracellular level of this purine metabolite compared with the initial (acute) value. Very similar results were obtained using a microdialysis technique that detected uric acid directly. These estimates of striatal uric acid concentration are in marked contrast to those obtained using 40-micron diameter carbon fiber electrodes, which showed a decrease from the acute preparation to less than 1 mumol/L under chronic conditions. Large values of h2 were also recorded with chronically implanted paste electrodes in the hippocampus and frontal cortex.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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R D O'Neill; J L Gonzalez-Mora; M G Boutelle; D E Ormonde; J P Lowry; A Duff; B Fumero; M Fillenz; M Mas |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of neurochemistry Volume: 57 ISSN: 0022-3042 ISO Abbreviation: J. Neurochem. Publication Date: 1991 Jul |
Date Detail:
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Created Date: 1991-07-23 Completed Date: 1991-07-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 22-9 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University College, Dublin, Ireland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / metabolism* Carbon / diagnostic use Dialysis Electrochemistry / methods* Electrodes, Implanted Extracellular Space / metabolism* Male Osmolar Concentration Rats Rats, Inbred Strains Time Factors Uric Acid / metabolism* |
| Chemical | |
Reg. No./Substance:
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69-93-2/Uric Acid; 7440-44-0/Carbon |
| Comments/Corrections | |
Comment In:
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J Neurochem. 1992 Aug;59(2):784-6
[PMID:
1378492
]
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Erratum In:
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J Neurochem 1991 Nov;57(5):1822 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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