Document Detail


Anomalous cell surface structure of sickle cell anemia erythrocytes as demonstrated by cell surface labeling and endo-beta-galactosidase treatment.
MedLine Citation:
PMID:  6799655     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Erythrocyte surface glycoproteins from patients with various types of sickle cell anemia have been analyzed and compared with those from normal individuals. By hemagglutination with various anti-carbohydrate antibodies, sickle cells showed profound increase of i antigens and moderate increase of GlcNAc beta 1 leads to 3Gal beta 1 leads to 3 Glc structure, whereas antigenicity toward globosidic structure was unchanged. In parallel to these findings, erythrocytes of sickle cell patients have additional sialylated lactosaminoglycan in Band 3. Thus, it can be concluded that erythrocytes of sickle cell patients are characterized by an altered cell surface structure which does not appear to be due to topographical changes of cell surface membrane. It is possible that the anemia or the "stress" hematopoiesis in these patients is responsible for these changes.
Authors:
M Fukuda; M N Fukuda; S Hakomori; T Papayannopoulou
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of supramolecular structure and cellular biochemistry     Volume:  17     ISSN:  0275-3723     ISO Abbreviation:  J Supramol Struct Cell Biochem     Publication Date:  1981  
Date Detail:
Created Date:  1982-04-12     Completed Date:  1982-04-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8106911     Medline TA:  J Supramol Struct Cell Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  289-97     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Anemia, Sickle Cell / blood*
Borohydrides
Carbohydrate Conformation
Carbohydrate Sequence
Electrophoresis, Polyacrylamide Gel
Erythrocyte Membrane / ultrastructure*
Erythrocytes / ultrastructure*
Galactosidases*
Glycoproteins / blood*
Glycoside Hydrolases*
Humans
Membrane Proteins / blood*
Tritium
beta-Galactosidase*
Grant Support
ID/Acronym/Agency:
CA19224/CA/NCI NIH HHS; CA29959/CA/NCI NIH HHS; GM23100/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Borohydrides; 0/Glycoproteins; 0/Membrane Proteins; 10028-17-8/Tritium; EC 3.2.1.-/Galactosidases; EC 3.2.1.-/Glycoside Hydrolases; EC 3.2.1.103/keratan-sulfate endo-1,4-beta-galactosidase; EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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