Document Detail

Annexin I and involucrin are cross-linked by particulate transglutaminase into the cornified cell envelope of squamous cell carcinoma Y1.
MedLine Citation:
PMID:  1348694     Owner:  NLM     Status:  MEDLINE    
The squamous cell carcinoma line, SqCC/Y1, like natural squamous epithelia, forms a cornified cell envelope during differentiation which can be directly correlated with an increase in particulate transglutaminase activity. When transglutaminase is activated in these cells by calcium ionophore X-537A, annexin I and involucrin become incorporated into the cornified cell envelope and cannot be extracted with solutions containing sodium dodecyl sulfate (SDS) and beta-mercaptoethanol. This effect is specific for annexin I; thus, the amounts of annexins II and IV that were extractable from cells by SDS and beta-mercaptoethanol did not change following treatment with ionophore X-537A. Annexin I could be cross-linked in vitro to itself and to other endogenous proteins by transglutaminase extracted from the particulate fraction of SqCC/Y1 cells. Immunofluorescence studies showed that cross-linked annexin I and involucrin form an envelope-like structure in SqCC/Y1 cells during differentiation that cannot be extracted by EGTA and Triton X-100. The amount of staining of this envelope structure corresponded directly to the particulate transglutaminase activity of these cells. Annexin I monoclonal and polyclonal antibodies were shown to bind to purified cornified cell envelopes from SqCC/Y1. These studies suggest that particulate transglutaminase regulates a function of annexin I during the differentiation of SqCC/Y1 cells by covalently cross-linking this protein into the cornified cell envelope.
K G Moore; A C Sartorelli
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  200     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1992 May 
Date Detail:
Created Date:  1992-05-18     Completed Date:  1992-05-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  186-95     Citation Subset:  IM    
Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06510.
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MeSH Terms
Calcium-Binding Proteins / chemistry*
Carcinoma, Squamous Cell / chemistry*
Cell Differentiation
Enzyme Induction
Membrane Proteins / analysis
Microscopy, Fluorescence
Protein Precursors / chemistry*
Transglutaminases / biosynthesis,  chemistry*
Grant Support
Reg. No./Substance:
0/Annexins; 0/Antibodies; 0/Calcium-Binding Proteins; 0/Membrane Proteins; 0/Protein Precursors; 60108-77-2/involucrin; EC

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