| Annatto extract and β-carotene enhances antioxidant status and regulate gene expression in neutrophils of diabetic rats. | |
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MedLine Citation:
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PMID: 22239725 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Annatto (Bixa orellanaL.) contain a mixture of orange-yellowish pigments due to the presence of various carotenoids that have antioxidant effect. The immune system is especially vulnerable to oxidative damage because many immune cells, such as neutrophils, produce reactive oxygen and nitrogen species as part of the body's defense mechanisms to destroy invading pathogens. It is well known that the function of neutrophils is altered in diabetes; one of the major functional changes in neutrophils in diabetes is the increased generation of extracellular superoxide via the NADPH oxidase system. The purpose of this study is to evaluate the production of ROS and NO as well as the expression of NADPH oxidase subunits, iNOS, superoxide dismutase and catalase in neutrophils from diabetic rats treated with annatto extract and β-carotene. Forty-eight female Fisher rats were distributed into six groups according to the treatment received. All animals were sacrificed 7 days after treatment and the neutrophils were isolated using two gradients of different densities. The ROS and NO were quantified by a chemiluminescence and spectrophotometric assays, respectively. Analyses of gene expression were performed using qRT-PCR. The results show that treatment with annatto extract and β-carotene was able to decrease ROS production and the mRNA levels of p22(phox) and p47(phox) and increase the mRNA levels of SOD and catalase in neutrophils from diabetic rats. These data suggest that annatto extract and β-carotene exerts antioxidant effect via inhibition of expression of the NADPH oxidase subunits and increase expression/activity of antioxidant enzymes. |
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Authors:
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Joamyr Victor Rossoni Júnior; Glaucy Rodrigues Araújo; Bruno da Cruz Pádua; Cíntia Lopes de Brito Magalhães; Míriam Martins Chaves; Maria Lúcia Pedrosa; Marcelo Eustáquio Silva; Daniela Caldeira Costa |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-12 |
Journal Detail:
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Title: Free radical research Volume: - ISSN: 1029-2470 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9423872 Medline TA: Free Radic Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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