| Anisoosmotic regulation of the Mi-2 autoantigen mRNA in H4IIE rat hepatoma cells and primary hepatocytes. | |
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MedLine Citation:
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PMID: 9755851 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Using the differential display polymerase chain reaction (DDRT-PCR) a 169 bp cDNA product, which is 88.8% homologous to the human Mi-2beta autoantigen, was identified in H4IIE rat hepatoma cells. At protein level 100% homology was found. The Mi-2 mRNA was downregulated after hypoosmotic exposure and upregulated after hyperosmotic exposure in H4IIE cells and rat hepatocytes. The human Mi-2 is an autoantigen in dermatomyositis and is a member of the SNF/RAD 54 helicase family. Accordingly, Mi-2 may not only be a target of osmosignalling but could also be involved in the osmosignalling pathway towards gene expression in H4IIE and liver parenchymal cells. |
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Authors:
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R Hammermann; U Warskulat; D Häussinger |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 435 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1998 Sep |
Date Detail:
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Created Date: 1998-10-05 Completed Date: 1998-10-05 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 21-4 Citation Subset: IM |
Affiliation:
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Medizinische Universitätsklinik der Heinrich-Heine-Universität Düsseldorf, Klinik für Gastroenterologie, Hepatologie und Infektiologie, Germany. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/X86691 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphatases* Amino Acid Sequence Animals Autoantigens / chemistry, genetics*, metabolism* Base Sequence Carcinoma, Hepatocellular / genetics*, immunology, metabolism* DNA Helicases / chemistry Liver / cytology, immunology, metabolism* Male Mi-2 Nucleosome Remodeling and Deacetylase Complex Molecular Sequence Data Polymerase Chain Reaction RNA, Messenger / metabolism* Rats Rats, Wistar Signal Transduction / genetics Tumor Cells, Cultured Water-Electrolyte Balance / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Autoantigens; 0/CHD4 protein, human; 0/RNA, Messenger; EC 3.5.1.98/Mi-2 Nucleosome Remodeling and Deacetylase Complex; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.1.-/DNA Helicases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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