Document Detail


Anion uptake and acid-base and ionic effects during isolated and combined exposure to hypercapnia and nitrite in the freshwater crayfish, Astacus astacus.
MedLine Citation:
PMID:  11128438     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nitrite influx into crayfish showed saturation kinetics, supporting a carrier-mediated uptake. Addition of 4,4'-diisothiocyanatostilbene-2,2'-disulfonate (DIDS: at 10(-5), 10(-4) and 10(-3) M) and bumetanide (at 10(-5) M and 10(-4) M) to the ambient water did not significantly affect nitrite influx. Rather than suggesting that neither Cl-/HCO3- exchange nor K+/Na+/2Cl- cotransport were involved in the transport, this may reflect that the gill cuticle has a low permeability to the pharmacological agents, or that the sensitivity of the transport mechanism to the inhibitors is low. Nitrite accumulation in the haemolymph was significantly decreased during hypercapnic conditions compared to normocapnic conditions. This supports the idea that an acid-base regulatory decrease in Cl-(influx)/HCO3- (efflux) induced by hypercapnia should decrease NO2- uptake if NO2- and Cl- share this uptake route. The respiratory acidosis caused by exposure to hypercapnia alone was partially compensated by HCO3- accumulation in the haemolymph. Combined exposure to hypercapnia and nitrite improved pH recovery, mainly by augmenting the [HCO3-] increase, but also by decreasing haemolymph PCO2. Exposure to nitrite in normocapnic water induced an initial increase in haemolymph [HCO3-] and later also a decrease in PCO2. Thus, the improved acid-base compensation during combined hypercapnia and nitrite exposure was an amplification of this nitrite-induced response. Haemolymph base excess rose much more than haemolymph [Ca], suggesting that transfer of acid-base equivalents between animal and water was more important than H+ buffering by exoskeletal CaCO3 in mediating the increase in haemolymph [HCO3-].
Authors:
F B Jensen; P Koldkjaer; A Bach
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology     Volume:  170     ISSN:  0174-1578     ISO Abbreviation:  J. Comp. Physiol. B, Biochem. Syst. Environ. Physiol.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-12-20     Completed Date:  2001-02-08     Revised Date:  2009-06-08    
Medline Journal Info:
Nlm Unique ID:  8413200     Medline TA:  J Comp Physiol B     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  489-95     Citation Subset:  IM    
Affiliation:
Institute of Biology, University of Southern Denmark, Odense University. fbj@biology.sdu.dk
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MeSH Terms
Descriptor/Qualifier:
Acid-Base Equilibrium / drug effects*
Animals
Anions / metabolism*
Astacoidea / metabolism*
Female
Hypercapnia / metabolism*
Ions
Kinetics
Male
Nitrites / pharmacokinetics,  pharmacology*
Chemical
Reg. No./Substance:
0/Anions; 0/Ions; 0/Nitrites

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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