| Animal models for prenatal gene therapy: the sheep model. | |
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MedLine Citation:
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PMID: 22648775 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Large animal experiments are vital in the field of prenatal gene therapy, to allow translation from small animals into man. Sheep provide many advantages for such experiments. They have been widely used in research into fetal physiology and pregnancy and the sheep fetus is a similar size to that in the human. Sheep are tolerant to in utero manipulations such as fetoscopy or even hysterotomy, and they are cheaper and easier to maintain than non-human primates. In this chapter, we describe the animal husbandry involved in generating time-mated sheep pregnancies, the large number of injection routes in the fetus that can be achieved using ultrasound or fetoscopic-guided injection, and laparotomy when these more minimally invasive routes of injection are not feasible. |
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Authors:
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Khalil N Abi-Nader; Michael Boyd; Alan W Flake; Vedanta Mehta; Donald Peebles; Anna L David |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 891 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2012 |
Date Detail:
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Created Date: 2012-05-31 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 219-48 Citation Subset: IM |
Affiliation:
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Prenatal Cell and Gene Therapy Group, EGA Institute for Women's Health, University College London, London, UK. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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