Document Detail


Anillin-related protein Mid1p coordinates the assembly of the cytokinetic contractile ring in fission yeast.
MedLine Citation:
PMID:  22918943     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In fission yeast cells cortical nodes containing the protein Blt1p and several kinases appear early in G2, mature into cytokinetic nodes by adding anillin Mid1p, myosin-II, formin Cdc12p, and other proteins, and condense into a contractile ring by movements that depend on actin and myosin-II. Previous studies concluded that cells without Mid1p lack cytokinetic nodes and assemble rings unreliably from myosin-II strands but left open questions. Why do strands form outside the equatorial region? Why is ring assembly unreliable without Mid1p? We found in Δmid1 cells that Cdc12p accumulates in cytokinetic nodes scattered in the cortex and produces actin filaments that associate with myosin-II, Rng2p, and Cdc15p to form strands located between the nodes. Strands incorporate nodes, and in ~67% of cells, strands slowly close into rings that constrict without the normal ~25-min maturation period. Ring assembly is unreliable and slow without Mid1p because the scattered Cdc12p nodes generate strands spread widely beyond the equator, and growing strands depend on random encounters to merge with other strands into a ring. We conclude that orderly assembly of the contractile ring in wild-type cells depends on Mid1p to recruit myosin-II, Rng2p, and Cdc15p to nodes and to place cytokinetic nodes around the cell equator.
Authors:
Shambaditya Saha; Thomas D Pollard
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-08-23
Journal Detail:
Title:  Molecular biology of the cell     Volume:  23     ISSN:  1939-4586     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-15     Completed Date:  2013-03-12     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3982-92     Citation Subset:  IM    
Affiliation:
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8103, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Polarity
Contractile Proteins / metabolism*
Cytokinesis*
Cytoskeletal Proteins / metabolism
Interphase
Mutation / genetics
Protein Interaction Mapping
Schizosaccharomyces / cytology*,  metabolism*
Schizosaccharomyces pombe Proteins / metabolism*
Grant Support
ID/Acronym/Agency:
GM-026338/GM/NIGMS NIH HHS; R01 GM026132/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Cdc12 protein, S pombe; 0/Contractile Proteins; 0/Cytoskeletal Proteins; 0/Mid1 protein, S pombe; 0/Schizosaccharomyces pombe Proteins; 0/anillin
Comments/Corrections

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