| Anillin Acts as a Bifunctional Linker Coordinating Midbody Ring Biogenesis during Cytokinesis. | |
| | |
MedLine Citation:
|
PMID: 22226749 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Animal cell cytokinesis proceeds via constriction of an actomyosin-based contractile ring (CR) [1, 2]. Upon reaching a diameter of ∼1 μm [3], a midbody ring (MR) forms to stabilize the intercellular bridge until abscission [4-6]. How MR formation is coupled to CR closure and how plasma membrane anchoring is maintained at this key transition is unknown. Time-lapse microscopy of Drosophila S2 cells depleted of the scaffold protein Anillin [7-9] revealed that Anillin is required for complete closure of the CR and formation of the MR. Truncation analysis revealed that Anillin N termini connected with the actomyosin CR and supported formation of stable MR-like structures, but these could not maintain anchoring of the plasma membrane. Conversely, Anillin C termini failed to connect with the CR or MR but recruited the septin Peanut to ectopic structures at the equatorial cortex. Peanut depletion mimicked truncation of the Anillin C terminus, resulting in MR-like structures that failed to anchor the membrane. These data demonstrate that Anillin coordinates the transition from CR to MR and that it does so by linking two distinct cortical cytoskeletal elements. One apparently acts as the core structural template for MR assembly, while the other ensures stable anchoring of the plasma membrane beyond the CR stage. |
| | |
Authors:
|
Amel Kechad; Silvana Jananji; Yvonne Ruella; Gilles R X Hickson |
Related Documents
:
|
18936319 - Arm raising at exposure-controlled multidetector trauma ct of thoracoabdominal region: ... 8551989 - Correcting for rf inhomogeneities in multiecho pulse sequence mri dosimetry. 21701109 - Hydrocephalus due to diffuse villous hyperplasia of the choroid plexus. 12558079 - Ct- and mr colonography. 9300759 - An analysis of the effect of ovoid shields in a selectron-ldr cervical applicator on do... 12625749 - Comparison of volume-rendered and surface-rendered mr colonography. 21121749 - Magnetic resonance imaging evaluation of adjacent segments after cervical disc arthropl... 12890829 - The objective diagnosis of early tennis elbow by magnetic resonance imaging. 3031969 - Radiographic evaluation of brachial plexopathy. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-1-4 |
Journal Detail:
|
Title: Current biology : CB Volume: - ISSN: 1879-0445 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
|
Created Date: 2012-1-9 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9107782 Medline TA: Curr Biol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2012 Elsevier Ltd. All rights reserved. |
Affiliation:
|
CHU Sainte-Justine Centre de Recherche, Centre de Cancérologie Charles Bruneau, 3175 Chemin de la Côte Ste-Catherine, Montréal, Québec H3T 1C5, Canada; Département de Pathologie et Biologie Cellulaire, Université de Montréal, Montréal, Québec H3C 3J7, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The RhoGAP Domain of CYK-4 Has an Essential Role in RhoA Activation.
Next Document: IFN-? producing T cells contribute to the increase of myeloid derived suppressor cells in tumor-bear...