Document Detail

Angular domain fluorescence imaging for small animal research.
MedLine Citation:
PMID:  20210468     Owner:  NLM     Status:  MEDLINE    
We describe a novel macroscopic fluorescent imaging technique called angular domain fluorescence imaging (ADFI) applicable to the detection of fluorophores embedded in biological tissues. The method exploits the collimation detection capabilities of an angular filter array (AFA). The AFA uses the principle of acceptance angle filtration to extract minimally scattered photons emitted from fluorophores deep within tissue. Our goal was to develop an ADFI system for imaging near-infrared fluorescent markers for small animal imaging. According to the experimental results, the ADFI system offered higher resolution and contrast compared to a conventional lens and lens-pinhole fluorescent detection system. Furthermore, ADFI of a hairless mouse injected with a fluorescent bone marker revealed vertebral structural and morphometric data that correlated well with data derived from volumetric x-ray computed tomography images. The results suggested that ADFI is a useful technique for submillimeter mapping of the distribution of fluorescent biomarkers in small animals.
Fartash Vasefi; Michelle Belton; Bozena Kaminska; Glenn H Chapman; Jeffrey J L Carson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  15     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:    2010 Jan-Feb
Date Detail:
Created Date:  2010-03-09     Completed Date:  2010-06-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  016023     Citation Subset:  IM    
Simon Fraser University, The School of Engineering Science, Burnaby, British Columbia, Canada.
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MeSH Terms
Diagnostic Imaging / methods*
Fluorescent Dyes / chemistry
Mice, Hairless
Phantoms, Imaging
Spectrometry, Fluorescence / methods*
Spine / anatomy & histology,  radiography
Tomography, X-Ray Computed
Reg. No./Substance:
0/Fluorescent Dyes

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