Document Detail

Angular dependent light scattering from multicellular spheroids.
MedLine Citation:
PMID:  11818017     Owner:  NLM     Status:  MEDLINE    
We demonstrate that the effects of cell-cell contact and of changes in cell shape have only a minor effect on the angular distribution of light scattering from mammalian fibroblast cells. This result is important for the development of light scattering as a noninvasive tool for tissue diagnostics such as cancer detection. Changes in cell organization that are not accompanied by changes in internal cellular structure may not be measurable. On the other hand, changes in internal cellular structure should be measurable without interference from changes in overall cellular organization. The second major result of this work is that there are small but significant differences between light scattering of tumorigenic and nontumorigenic cells grown in a three-dimensional culture system. The cause of the differences in light scattering are likely due to the nontumorigenic cells arresting in the G1 phase of the cell cycle, while the tumorigenic cells continue to proliferate.
J R Mourant; T M Johnson; V Doddi; J P Freyer
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  7     ISSN:  1083-3668     ISO Abbreviation:  J Biomed Opt     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-01-30     Completed Date:  2002-04-30     Revised Date:  2012-10-09    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  93-9     Citation Subset:  IM    
Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico 87545, USA.
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MeSH Terms
Cell Count
Cell Cycle
Neoplasms / diagnosis*,  pathology,  physiopathology*,  ultrastructure
Reference Values
Scattering, Radiation
Spheroids, Cellular / cytology,  pathology,  radiation effects*,  ultrastructure
Grant Support

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