Document Detail


Anguilla anguilla L. liver ethoxyresorufin O-deethylation, glutathione S-transferase, erythrocytic nuclear abnormalities, and endocrine responses to naphthalene and beta-naphthoflavone.
MedLine Citation:
PMID:  12706398     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of naphthalene (NAP) and beta-naphthoflavone (BNF) on phase I biotransformation and genotoxicity in Anguilla anguilla L. were evaluated. Phase II biotransformation and cortisol levels were also assessed in NAP-treated fish. Two groups of eels were exposed to either a NAP or a BNF concentration range (0.1-2.7 microM) for different exposure periods (2-72 h). An early significant ethoxyresorufin O-deethylation (EROD) activity inhibition was observed, especially for the highest NAP concentrations at 2-6 h exposure and for BNF at 2h exposure. However, a significant EROD activity increase was detected from 16 to 72 h exposure for NAP and from 4 to 72 h exposure for BNF. The cytochrome P450 (P450) content was not dose related. However, with regard to BNF exposure, P450 was the first biomarker to respond. Liver alanine transaminase (ALT) activity was measured as an indicator of hepatic health condition. ALT results demonstrated that the EROD activity decrease, previously described for NAP, was not related to tissue damage. Nevertheless, the highest BNF concentrations were demonstrated to induce liver damage and to impair the EROD activity response. An increased genotoxic response, measured as erythrocytic nuclear abnormalities (ENA), was observed during the first 8h NAP exposure. However, for exposures longer than 8 h, ENA frequency returned to the control levels. This response profile may reflect a considerable DNA repair capacity and/or a metabolic adaptation providing an efficient NAP biotransformation and consequent detoxification. BNF revealed no ENA alterations for all concentrations and exposure lengths. In the NAP experiment a causal relationship between immature erythrocytes (IE) and ENA frequency disappearance was not found. BNF results with regard to IE frequency revealed an ability to alter the balance between erythropoiesis and removal of erythrocytes. Liver glutathione S-transferase activity was significantly induced after 2 and 48 h NAP exposure. A cortisol-impaired response seems to occur from 4 to 24 h NAP exposure, demonstrating an endocrine disruption. However, an adaptation process seems to occur after 48 h, since the plasma cortisol had a tendency to increase. The present findings confirm the usefulness of the adopted biomarkers. The ecological risk associated with aquatic contamination by NAP was also confirmed by the present data.
Authors:
M Teles; M Pacheco; M A Santos
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Ecotoxicology and environmental safety     Volume:  55     ISSN:  0147-6513     ISO Abbreviation:  Ecotoxicol. Environ. Saf.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-04-22     Completed Date:  2003-05-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7805381     Medline TA:  Ecotoxicol Environ Saf     Country:  United States    
Other Details:
Languages:  eng     Pagination:  98-107     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Anguilla / physiology*
Animals
Cell Nucleus / pathology
Cytochrome P-450 CYP1A1 / drug effects,  pharmacology*
DNA Damage*
DNA Repair
Endocrine System / drug effects
Enzyme Inhibitors / adverse effects*
Erythrocytes
Glutathione Transferase / drug effects,  pharmacology*
Liver / enzymology
Naphthalenes / adverse effects*
Water Pollutants, Chemical / adverse effects*
beta-Naphthoflavone / adverse effects*
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Naphthalenes; 0/Water Pollutants, Chemical; 6051-87-2/beta-Naphthoflavone; 91-20-3/naphthalene; EC 1.14.14.1/Cytochrome P-450 CYP1A1; EC 2.5.1.18/Glutathione Transferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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