| Angiotensinogen gene polymorphism of threonine/methionine at position 235-potential problems of the modified restriction endonuclease (Tth111-I) digestion method. | |
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MedLine Citation:
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PMID: 9442444 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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There are four methods for detecting the angiotensinogen gene Agt T235/M235; 1) allele-specific oligonucleotide hybridization (PCR-ASO); 2) mutagenically separated PCR (MS-PCR) using three primers to amplify all possible alleles in on PCR reaction; 3) restriction endonuclease Tth111-I; 4) SfaN-I digestion method using mismatched primer for the PCR (modified PCR-RFLP). Two of these four methods have been used in Japanese studies. The reported allelic frequencies of Agt T235/M235 in normal controls in the Japanese population are around 0.75-0.84/0.25-0.16 (as a whole 0.80/0.20) by PCR-ASO and 0.70-0.65/0.30-0.35 (as a whole 0.67/0.33) by the modified PCR-RFLP (Tth111-I). The present study tested how these methods contribute to the differences in Agt T235/M235. By PCR-ASO, the genotypes could be clearly determined. However, it is hard to complete every digestive reaction under the experimental conditions described for modified PCR-RFLP (Tth111-I). Thus, for studying Agt T235/M235, PCR-ASO or some method other than PCR-RFLP (Tth111-I) can be recommended. Our findings suggest that the allele frequency of Agt T235/M235 in the normal Japanese population is closer to 0.75-0.84/0.25-0.16. Although a strong association was reported between the Agt T235 allele and essential hypertension or myocardial infarction, using the modified PCR-RFLP (Tth111-I), two of three studies using PCR-ASO found no or only a weak association. The relationship between the Agt T235 allele and essential hypertension or myocardial infarction in the Japanese population needs to be assessed. |
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Authors:
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N Kawada; T Moriyama; E Imai; M Hori |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of nephrology Volume: 10 ISSN: 1121-8428 ISO Abbreviation: J. Nephrol. Publication Date: 1997 Nov-Dec |
Date Detail:
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Created Date: 1998-02-26 Completed Date: 1998-02-26 Revised Date: 2008-08-29 |
Medline Journal Info:
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Nlm Unique ID: 9012268 Medline TA: J Nephrol Country: ITALY |
Other Details:
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Languages: eng Pagination: 314-7 Citation Subset: IM |
Affiliation:
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First Department of Medicine, Osaka University School of Medicine, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Angiotensinogen
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genetics* Deoxyribonucleases, Type II Site-Specific / metabolism* Humans Methionine Polymerase Chain Reaction / methods* Polymorphism, Restriction Fragment Length* Threonine |
| Chemical | |
Reg. No./Substance:
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11002-13-4/Angiotensinogen; 63-68-3/Methionine; 72-19-5/Threonine; EC 3.1.21.4/Deoxyribonucleases, Type II Site-Specific; EC 3.1.21.4/TCGA-specific type II deoxyribonucleases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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