Document Detail


Angiotensin receptors in hormone-independent prostate cancer cell line DU145: presence of two variants of angiotensin type 1 receptor.
MedLine Citation:
PMID:  19333191     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Recently, the involvement of the renin-angiotensin system in the proliferation of prostate cancer has been suggested. There is increasing evidence that angiotensin II receptor type 1 (AT1) is expressed in a variety of cancer cells and tissues and may have a role in tumor growth, angiogenesis, and invasive activity of malignant lesions in vivo. The implications of data referring to an angiotensin receptor in hormone-independent human prostate cancer DU145 cells are unclear. Angiotensin II has been shown to inhibit the proliferation of DU145 cell lines. However, it is known that AT1 stimulates cell proliferation and that angiotensin II receptor type 2 (AT2) induces apoptosis and inhibits cell proliferation. MATERIAL/METHODS: The aim of our study was to investigate, by means of immunohistochemical and reverse transcriptase polymerase chain reaction assays, the type of angiotensin II receptor that is present in DU145 cells. RESULTS: In DU145-derived complementary deoxyribonucleic acid (cDNA), a polymerase chain reaction assay revealed 2 AT1-specific PCR products (93 bp and 126 bp). DU145-derived cDNA did not reveal AT2 expression at a level sufficient for detection by PCR. In cultured cells, immunohistochemical testing revealed a positive reaction in cultures immunostained with anti-AT1 antibody but not in those immunostained with AT2 antibody. CONCLUSIONS: The inhibitory effect of angiotensin II on the proliferation of DU145 cells is exerted via AT1. It is possible that presence of 2 variants of AT1 in that cancer cell line is essential to produce the biological effects of angiotensin.
Authors:
Małgorzata Sidorkiewicz; Elzbieta Rebas; Marta Szymajda; Hanna Ławnicka; Marek Pawlikowski; Agnieszka Lachowicz
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medical science monitor : international medical journal of experimental and clinical research     Volume:  15     ISSN:  1643-3750     ISO Abbreviation:  Med. Sci. Monit.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-31     Completed Date:  2009-05-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9609063     Medline TA:  Med Sci Monit     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  BR106-10     Citation Subset:  IM    
Affiliation:
Department of Medical Biochemistry, Medical University of Łódź, Łódź, Poland.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis
Base Sequence
Cell Line, Tumor
Cell Proliferation
DNA Primers
DNA, Complementary
Humans
Immunohistochemistry
Male
Prostatic Neoplasms / genetics,  metabolism*,  pathology
Receptor, Angiotensin, Type 1 / genetics,  metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Complementary; 0/Receptor, Angiotensin, Type 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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