Document Detail

Angiotensin-converting enzyme inhibition and free-radical scavenging properties of cationic peptides derived from soybean protein hydrolysates.
MedLine Citation:
PMID:  18636366     Owner:  NLM     Status:  MEDLINE    
Soybean protein isolate was hydrolyzed consecutively with pepsin and pancreatin followed by separation of the digest on a SP-Sepharose column to obtain angiotensin-converting enzyme (ACE)-inhibitory fractions. Three of the five fractions obtained inhibited ACE activity, with values of the concentration of hydrolysate fraction that inhibited 50% of ACE activity of 1.09, 0.42, and 0.25 mg/ml for fractions 2, 3, and 4, respectively. Kinetic analysis showed that fractions 2, 3, and 4 are non-competitive, competitive and mixed inhibitors of ACE, respectively. Fluorescence emission of ACE was quenched along with a red shift in wavelength of emission maxima in the presence of the protein hydrolysate fractions. Fraction 3 was the most potent free-radical scavenger, while fraction 4 had almost no scavenging effect. We concluded that fraction 3 had the most potential to be used as a bioactive ingredient because of its strong ACE-inhibitory and free-radical scavenging properties.
Vahid Farzamirad; Rotimi E Aluko
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of food sciences and nutrition     Volume:  59     ISSN:  0963-7486     ISO Abbreviation:  Int J Food Sci Nutr     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-11-03     Completed Date:  2009-01-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9432922     Medline TA:  Int J Food Sci Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  428-37     Citation Subset:  IM    
Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba, Canada.
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MeSH Terms
Angiotensin-Converting Enzyme Inhibitors / analysis*,  pharmacology
Free Radical Scavengers / analysis*,  pharmacology
Microscopy, Fluorescence
Pancreatin / metabolism
Pepsin A / metabolism
Protein Hydrolysates / chemistry*
Soybean Proteins / chemistry*
Spectrophotometry / methods
Reg. No./Substance:
0/Angiotensin-Converting Enzyme Inhibitors; 0/Cations; 0/Free Radical Scavengers; 0/Protein Hydrolysates; 0/Soybean Proteins; 8049-47-6/Pancreatin; EC A

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