| Angiotensin II (A-II) steroidogenic refractoriness in Y-1 cells in the presence of A-II receptors negatively coupled to adenylate cyclase. | |
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MedLine Citation:
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PMID: 2828018 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Y1 adrenal tumor cells are resistant to the steroidogenic effect of A-II though they possess specific A-II binding sites. The number of these binding sites is lower in Y1 cells than in bovine adrenal cells, but the affinity is similar in the two models. Moreover, Y1 cells are shown to contain a high level of cytosolic protein kinase C whose properties appear similar to those observed in bovine adrenal cells. However, the activation of protein kinase C by a phorbol ester (PMA) or diacylglycerol (OAG) does not induce steroidogenesis in Y1 cells. On the other hand, A-II, without any effect on adenylate cyclase in basal conditions, reduces the ACTH-induced cAMP production in Y1 cells. This inhibitory effect of A-II is not blocked by phosphodiesterase inhibitor but is completely abolished after 24 hours of pretreatment of intact cells with pertussis toxin. This inhibition is probably mediated by the inhibitory guanine nucleotide regulatory protein (Gi) since the labeled 41 KD-ADP ribosylated protein disappeared after 24 hours of pretreatment of intact cells with pertussis toxin. Moreover, the accumulation of inositol phosphates under A-II stimulation was low, which suggests that the coupling of A-II receptors with phospholipase C is reduced in Y1 cells. The Y1 cell line is probably a good model to study the post membrane events in A-II action. |
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Authors:
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M Begeot; D Langlois; I Vilgrain; J M Saez |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Endocrine research Volume: 13 ISSN: 0743-5800 ISO Abbreviation: Endocr. Res. Publication Date: 1987 |
Date Detail:
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Created Date: 1988-03-15 Completed Date: 1988-03-15 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8408548 Medline TA: Endocr Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 301-16 Citation Subset: IM |
Affiliation:
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INSERM U 307, Hôpital Debrousse, Lyon, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenylate Cyclase
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metabolism Adenylate Cyclase Toxin Adrenal Cortex / drug effects, metabolism* Adrenal Cortex Hormones / biosynthesis* Adrenocorticotropic Hormone / pharmacology Angiotensin II / antagonists & inhibitors, metabolism, pharmacology* Animals Calcium / physiology Cells, Cultured Cyclic AMP / biosynthesis Inositol Phosphates / metabolism Mice Pertussis Toxin Protein Kinase C / metabolism Radioligand Assay Receptors, Angiotensin / metabolism Tetradecanoylphorbol Acetate / pharmacology Virulence Factors, Bordetella / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Adenylate Cyclase Toxin; 0/Adrenal Cortex Hormones; 0/Inositol Phosphates; 0/Receptors, Angiotensin; 0/Virulence Factors, Bordetella; 11128-99-7/Angiotensin II; 16561-29-8/Tetradecanoylphorbol Acetate; 60-92-4/Cyclic AMP; 7440-70-2/Calcium; 9002-60-2/Adrenocorticotropic Hormone; EC 2.4.2.31/Pertussis Toxin; EC 2.7.11.13/Protein Kinase C; EC 4.6.1.1/Adenylate Cyclase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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