Document Detail

Angiotensin II release from rabbit intrarenal arteries: a critical assessment.
MedLine Citation:
PMID:  9056688     Owner:  NLM     Status:  MEDLINE    
A microdissected rabbit intrarenal arterial network (IAN) perfused at constant flow with Krebs-bicarbonate solution was employed to determine whether this network, which has a high renin content, releases angiotensin II (AII) either spontaneously or during beta-adrenergic stimulation. Six groups of experiments were conducted in which samples of the vascular effluent were collected on Sep Paks before and during intraluminal infusion of L-isoproterenol (1.1 to 11 microg/min). Separation and assay of AII were by combined HPLC and RIA. For an accurate estimation of the quantity of AII released, it was important to subtract the Krebs and isoproterenol blanks, 19 and 23 pg, respectively, from the basal and isoproterenol-induced AII release. In Groups 1 and 2, AII release was determined before and during isoproterenol infusion (5.5 microg/min). Basal release of AII was insignificant in Groups 1 to 5. In Group 1, infusion of isoproterenol caused AII release from IAN before and after removal of glomeruli (glomerulectomy), but with variability between experiments. An even higher infusion rate of isoproterenol (11 microg/min) in Group 2 caused no significant AII release. Similarly, in Group 3, in which a longer collection period was imposed, isoproterenol (5.5 microg/min) failed to cause significant AII release. In Groups 4 and 5, Goldblatt hypertensive and salt-restricted rabbits, respectively, isoproterenol caused AII release, but the effect was statistically significant only in Group 4. Supplying renin substrate in Group 6 caused only a small spontaneous AII release. We conclude that under these conditions of complete isolation from the intact circulation, the IAN despite a high renin content, releases little locally generated AII.
B G Zimmerman; P C Birt
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of hypertension     Volume:  10     ISSN:  0895-7061     ISO Abbreviation:  Am. J. Hypertens.     Publication Date:  1997 Mar 
Date Detail:
Created Date:  1997-06-04     Completed Date:  1997-06-04     Revised Date:  2009-02-24    
Medline Journal Info:
Nlm Unique ID:  8803676     Medline TA:  Am J Hypertens     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  306-14     Citation Subset:  IM    
Department of Pharmacology, University of Minnesota, Minneapolis 55455, USA.
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MeSH Terms
Adrenergic beta-Agonists / pharmacology
Angiotensin II / metabolism*
Arteries / metabolism*,  pathology
Chromium Radioisotopes / diagnostic use
Hypertension, Renovascular / metabolism,  pathology,  physiopathology
Hypertrophy / pathology
Isoproterenol / pharmacology
Kidney / metabolism*,  pathology
Renal Circulation / physiology*
Renin / metabolism
Sodium / deficiency
Grant Support
Reg. No./Substance:
0/Adrenergic beta-Agonists; 0/Chromium Radioisotopes; 11128-99-7/Angiotensin II; 7440-23-5/Sodium; 7683-59-2/Isoproterenol; EC

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