Document Detail

Angiotensin II induces apoptosis in human mural granulosa-lutein cells, but not in cumulus cells.
MedLine Citation:
PMID:  18684452     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To test whether angiotensin II (AngII) could modulate apoptosis of human granulosa-lutein (GL) cells from gonadotropin-stimulated follicles. DESIGN: In vitro assays on mural and cumulus granulosa cells. SETTING: University laboratory and private IVF practice. PATIENT(S): One hundred six consecutive women undergoing 113 IVF cycles. INTERVENTION(S): Purified human GL mural or cumulus cells were cultured in serum-free media in the presence or absence of AngII with or without the AngII receptor blockers saralasin and CGP42112A. MAIN OUTCOME MEASURE(S): Detection of apoptosis using a fluorescent in situ marker for activated caspases. RESULT(S): Mural cells had approximately eightfold the amount of apoptosis compared with cumulus cells (average 0.23 vs. <0.03, respectively). With mural cells, AngII increased GL cell apoptosis versus untreated control samples (AngII 10(-)11 mol/L +6.5%; AngII 10(-9) mol/L +13.3%, and AngII 10(-7) mol/L +11.3%), an effect which was blocked by concurrent incubation with AngII receptor blockers. The AngII receptor blockers produced a significant decrease of apoptosis compared with control cultures (saralasin: 19.4%; CGP42112A: 28.9%). Neither AngII nor blockers had effect on cumulus cells. CONCLUSION(S): Preovulatory concentrations of AngII, most likely via AT2 receptors, increase apoptosis of cultured mural GL cells but have no effect on cumulus cells. Granulosa cells appear to be differentially regulated by AngII.
Elisa Acosta; Oscar Peña; Frederick Naftolin; Julio Avila; Angela Palumbo
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-05
Journal Detail:
Title:  Fertility and sterility     Volume:  91     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-28     Completed Date:  2009-05-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1984-9     Citation Subset:  IM    
Department of Biochemistry and Molecular Biology, University of La Laguna, Tenerife, Spain.
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MeSH Terms
Aging / physiology
Angiotensin II / pharmacology*
Angiotensin II Type 1 Receptor Blockers / pharmacology
Apoptosis / drug effects*
Cell Culture Techniques
Culture Media, Serum-Free
Cumulus Cells / cytology*,  drug effects,  physiology
Fertilization in Vitro
Follicle Stimulating Hormone / blood
Granulosa Cells / cytology*,  drug effects,  physiology
Menstrual Cycle
Ovulation Induction
Patient Selection
Receptor, Angiotensin, Type 1 / drug effects,  physiology
Young Adult
Reg. No./Substance:
0/Angiotensin II Type 1 Receptor Blockers; 0/Culture Media, Serum-Free; 0/Receptor, Angiotensin, Type 1; 11128-99-7/Angiotensin II; 9002-68-0/Follicle Stimulating Hormone

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