| Angiotensin II activates NADPH oxidase in isolated rat hearts subjected to ischaemia-reperfusion. | |
| | |
MedLine Citation:
|
PMID: 12591107 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The role of angiotensin II in myocardial ischaemia-reperfusion is not clearly defined. In this respect, the involvement of NADPH oxidase remains to be determined. The aim of this study was 1) to evaluate the cardiac effects of angiotensin AT(1) receptor stimulation in non-ischaemic conditions of perfusion or during ischaemia-reperfusion, and 2) to measure the concomitant activation of NADPH oxidase in isolated rat hearts perfused with angiotensin II and/or Losartan. In non-ischaemic hearts, angiotensin II induced rapid and prolonged vasoconstrictive and negative inotropic effects. Ischaemia-reperfusion increased the mRNA expression of AT(1) and AT(2) receptors. During reperfusion, angiotensin II reduced the incidence of arrhythmias and the lactate dehydrogenase released, and increased NADPH oxidase mRNA expression and enzyme activity. Losartan co-administration totally antagonised the effects of angiotensin II. Our study demonstrates that ischaemia-reperfusion induces adaptative cardiac modifications, which allow exogenously added angiotensin II to stimulate myocardial NADPH oxidase through angiotensin AT(1) receptor activation. |
| | |
Authors:
|
Alexandra Oudot; Catherine Vergely; Aline Ecarnot-Laubriet; Luc Rochette |
Related Documents
:
|
3180357 - Superoxide dismutase conjugated to polyethylene glycol provides sustained protection ag... 11605997 - Nifedipine limits infarct size via no-dependent mechanisms in dogs. 2297827 - Occurrence of oxidative stress during reperfusion of the human heart. 3993527 - Lack of correlation between occlusion and reperfusion arrhythmias in the cat. 23367457 - Assessment of hemodynamic load components affecting optimization of cardiac resynchroni... 1244257 - Echocardiographic spectrum of ebstein's anomaly of the tricuspid valve. |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: European journal of pharmacology Volume: 462 ISSN: 0014-2999 ISO Abbreviation: Eur. J. Pharmacol. Publication Date: 2003 Feb |
Date Detail:
|
Created Date: 2003-02-19 Completed Date: 2003-07-02 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 1254354 Medline TA: Eur J Pharmacol Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 145-54 Citation Subset: IM |
Affiliation:
|
Laboratoire de Physiopathologie et Pharmacologie Cardio-vasculaires Expérimentales, Faculties of Medicine and Pharmacy, 7, Boulevard Jeanne d'Arc, BP 87900, 21079 Dijon, France. aoudot@hotmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Angiotensin II
/
pharmacology* Animals Arrhythmias, Cardiac / physiopathology, prevention & control Coronary Circulation / drug effects Enzyme Activation / drug effects Heart / drug effects*, physiology, physiopathology L-Lactate Dehydrogenase / drug effects, metabolism Losartan / pharmacology Male Membrane Glycoproteins / genetics Membrane Transport Proteins* Myocardial Ischemia / complications* Myocardial Reperfusion Injury / etiology, physiopathology*, prevention & control Myocardium / enzymology*, metabolism NADPH Dehydrogenase / genetics NADPH Oxidase / genetics, metabolism* Perfusion Phosphoproteins / genetics RNA, Messenger / drug effects, genetics, metabolism Rats Rats, Wistar Vasoconstrictor Agents / pharmacology Ventricular Function, Left / drug effects, physiology |
| Chemical | |
Reg. No./Substance:
|
0/CYBB protein, human; 0/Membrane Glycoproteins; 0/Membrane Transport Proteins; 0/Phosphoproteins; 0/RNA, Messenger; 0/Vasoconstrictor Agents; 11128-99-7/Angiotensin II; 114798-26-4/Losartan; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.6.3.1/CYBA protein, human; EC 1.6.3.1/NADPH Oxidase; EC 1.6.99.1/NADPH Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of a single dose aspirin on platelets in humans with multiple risk factors for coronary arter...
Next Document: The effect of chronic renal failure on phosphodiesterase inhibitor-induced relaxation responses in r...