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Angiotensin-(1-7) Treatment Ameliorates Angiotensin II-Induced HUVEC Apoptosis.
MedLine Citation:
PMID:  23030315     Owner:  NLM     Status:  Publisher    
Angiotensin-(1-7) (Ang-(1-7)), a metabolite of angiotensin I and angiotensin II (Ang II), is a counterregulatory mediator of Ang II. Here, we investigated the effects of Ang-(1-7) on Ang II-induced apoptosis in human umbilical vein endothelial cells (HUVECs). Acridine orange/ethidium bromide (AO/EB) staining and propidium iodide (PI) staining were used to analyse the effect of Ang-(1-7) on Ang II-induced apoptosis. Ang-(1-7) alone did not affect the apoptosis of HUVECs. Ang II significantly enhanced the numbers of apoptotic cells (P<0.01). Ang-(1-7) suppressed Ang II-induced apoptosis. A-779, the specific receptor antagonist of Ang-(1-7), almost completely abolished the anti-apoptotic effects of Ang-(1-7). Moreover, Ang-(1-7) also inhibited Ang II-induced accumulation of cleaved caspase-3 and enhanced the expression of anti-apoptotic factor, B-cell lymphoma 2 (Bcl-2) at both mRNA and protein levels. The effect of Ang-(1-7) on Ang II-induced upregulation of lectin-like oxidised low-density lipoprotein receptor-1 (LOX-1), which is involved in endothelial apoptosis, was also analysed at both mRNA and protein levels Similarly, Ang-(1-7) dose dependently diminished Ang II-induced LOX-1 expression at both mRNA and protein levels. A-779 almost completely reversed Ang-(1-7)-mediated inhibition of Ang II-induced upregulation of LOX-1. LOX-1 silencing by small interfering RNAs (siRNAs) enhanced the protective effects of Ang-(1-7) against Ang-II-induced apoptosis in HUVECs. Taken together, our data suggested that Ang-(1-7) can ameliorate Ang II-induced apoptosis of HUVECs, at lease partially through suppression of LOX-1 expression. © 2012 The Authors Clinical and Experimental Pharmacology and Physiology © 2012 Wiley Publishing Asia Pty Ltd.
Hui-Yu Yang; Yun-Fei Bian; Hua-Ping Zhang; Fen Gao; Chuan-Shi Xiao; Bin Liang; Jin Li; Na-Na Zhang; Zhi-Ming Yang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-3
Journal Detail:
Title:  Clinical and experimental pharmacology & physiology     Volume:  -     ISSN:  1440-1681     ISO Abbreviation:  Clin. Exp. Pharmacol. Physiol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0425076     Medline TA:  Clin Exp Pharmacol Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 The Authors Clinical and Experimental Pharmacology and Physiology © 2012 Wiley Publishing Asia Pty Ltd.
Department of Cardiology, The Second Hospital of Shanxi Medical University, Taiyuan, China.
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