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Angiographic evaluation of the rat carotid balloon injury model.
MedLine Citation:
PMID:  21798260     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
RATIONALE: The rat carotid balloon-injury (BI) model is a widely used model of intimal hyperplasia (IH) and vascular remodeling. A variable degree of IH after BI has been previously reported, and we have encountered technical challenges and suboptimal results with the original method. OBJECTIVE: To evaluate the original rat carotid artery BI method with the use of micro-angiography. We tested the hypothesis that in order to obtain an optimal arterial response, BI should be limited to the common carotid artery with preservation of blood flow. METHODS AND RESULTS: The left common carotid artery (CCA) was injured by one of three different methods. Carotid angiograms and pathology were examined 14days after BI. A 2F Fogarty balloon catheter inflated to 2atm inside the aortic arch would not slide back into the common carotid artery until deflation to 0.5 to 0.7atm. Four out of five (80%) vessels injured with this method developed excessive inflammation without discernible IH. Six out of nine (66%) arteries that underwent BI limited to the CCA at 2atm developed the largest angiographic stenosis (p=0.003) and IH (0.20±0.03mm(2), p=0.028). Ten out of eleven (91%) arteries injured with a variable pressure of 1.5 to 2.2atm, based on the operator's feedback, developed considerable IH (0.12±0.02mm(2)). All injured carotid arteries with preserved blood flow on angiography developed IH with intact histological boundaries. CONCLUSIONS: Optimal IH with preservation of histological boundaries is achieved by graded BI limited to the CCA that preserves carotid blood flow.
Authors:
Ludwig D Orozco; Huiling Liu; Betty B Chen; Jonathan D Fratkin; Eddie Perkins
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-25
Journal Detail:
Title:  Experimental and molecular pathology     Volume:  -     ISSN:  1096-0945     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370711     Medline TA:  Exp Mol Pathol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Inc.
Affiliation:
University of Mississippi Medical Center, Department of Neurosurgery, 2500 N State Street Jackson, MS 39216, USA.
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