| Angiogenic potential of human dental pulp stromal (stem) cells. | |
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MedLine Citation:
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PMID: 19822086 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dental pulp is a heterogeneous microenviroment where unipotent progenitor and pluripotent mesenchymal stem cells cohabit. In this study we investigated whether human dental pulp stromal (stem) cells (DP-SCs) committed to the angiogenic fate. DP-SCs showed the specific mesenchymal immunophenotypical profile positive for CD29, CD44, CD73, CD105, CD166 and negative for CD14, CD34, CD45, in accordance with that reported for bone marrow-derived SCs. The Oct-4 expression in DP-SCs, evaluated through RT-PCR analysis, increased in relation with the number of the passages in cell culture and decreased after angiogenic induction. In agreement with their multipotency, DP-SCs differentiated toward osteogenic and adipogenic commitments. In angiogenic experiments, differentiation of DP-SCs, through vascular endothelial growth factor (VEGF) induction, was evaluated by in vitro matrigel assay and by cytometric analysis. Accordingly, endothelial-specific markers like Flt-1 and KDR were basally expressed and they increased after exposure to VEGF together with the occurrence of ICAM-1 and von Willebrand factor positive cells. In addition, VEGF-induced DP-SCs maintained endothelial cell-like features when cultured in a 3-D fibrin mesh, displaying focal organization into capillary-like structures. The DP-SC angiogenic potential may prove a remarkable tool for novel approaches to developing tissue-engineered vascular grafts which are useful when vascularization of ischemic tissues is required. |
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Authors:
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C Marchionni; L Bonsi; F Alviano; G Lanzoni; A Di Tullio; R Costa; M Montanari; P L Tazzari; F Ricci; G Pasquinelli; C Orrico; A Grossi; C Prati; G P Bagnara |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of immunopathology and pharmacology Volume: 22 ISSN: 0394-6320 ISO Abbreviation: Int J Immunopathol Pharmacol Publication Date: 2009 Jul-Sep |
Date Detail:
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Created Date: 2009-10-13 Completed Date: 2009-12-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8911335 Medline TA: Int J Immunopathol Pharmacol Country: Italy |
Other Details:
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Languages: eng Pagination: 699-706 Citation Subset: IM |
Affiliation:
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Department of Histology, Embryology and Applied Biology, University of Bologna, and Transfusion Medicine Service, S. Orsola Hospital, Bologna, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Adult Stem Cells / immunology, metabolism, physiology* Biological Markers / metabolism Cell Differentiation Cell Lineage Cell Separation Cells, Cultured Dental Pulp / cytology, immunology, metabolism, physiology* Endothelial Cells / immunology, metabolism, physiology* Fibrin / metabolism Flow Cytometry Humans Immunophenotyping Intercellular Adhesion Molecule-1 / metabolism Male Mesenchymal Stem Cells / physiology* Microscopy, Electron, Transmission Neovascularization, Physiologic* Octamer Transcription Factor-3 / genetics RNA, Messenger / metabolism Receptors, Vascular Endothelial Growth Factor / metabolism Stromal Cells / immunology, metabolism, physiology* Tissue Engineering* Vascular Endothelial Growth Factor A / metabolism von Willebrand Factor / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Octamer Transcription Factor-3; 0/POU5F1 protein, human; 0/RNA, Messenger; 0/Vascular Endothelial Growth Factor A; 0/von Willebrand Factor; 126547-89-5/Intercellular Adhesion Molecule-1; 9001-31-4/Fibrin; EC 2.7.10.1/Receptors, Vascular Endothelial Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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