Document Detail


Angiogenic growth factor expression in rat skeletal muscle in response to exercise training.
MedLine Citation:
PMID:  12543634     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Angiogenesis occurs in skeletal muscle in response to exercise training. To gain insight into the regulation of this process, we evaluated the mRNA expression of factors implicated in angiogenesis over the course of a training program. We studied sedentary control (n = 17) rats and both sedentary (n = 18) and exercise-trained (n = 48) rats with bilateral femoral artery ligation. Training consisted of treadmill exercise (4 times/day, 1-24 days). Basal mRNA expression in sedentary control muscle was inversely related to muscle vascularity. Angiogenesis was histologically evident in trained white gastrocnemius muscle by day 12. Training produced initial three- to sixfold increases in VEGF, VEGF receptors (KDR and Flt), the angiopoietin receptor (Tie-2), and endothelial nitric oxide synthase mRNA, which dissipated before the increase in capillarity, and a substantial (30- to 50-fold) but transient upregulation of monocyte chemoattractant protein 1 mRNA. These results emphasize the importance of early events in regulating angiogenesis. However, we observed a sustained elevation of the angiopoietin 2-to-angiopoietin 1 ratio, suggesting continued vascular destabilization. The response to exercise was (in general) tempered in high-oxidative muscles. These findings place importance on cellular events coupled to the onset of angiogenesis.
Authors:
Pamela G Lloyd; Barry M Prior; Hsiao T Yang; Ronald L Terjung
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.     Date:  2003-01-23
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  284     ISSN:  0363-6135     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-04-07     Completed Date:  2003-05-15     Revised Date:  2011-08-03    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H1668-78     Citation Subset:  IM    
Affiliation:
Department of Biomedical Sciences, College of Veterinary Medicine, University of Missouri, 1600 E. Rollins Road, Columbia, MO 65211, USA.
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MeSH Terms
Descriptor/Qualifier:
Angiogenesis Inducing Agents / genetics
Angiopoietin-1
Angiopoietin-2
Animals
Capillaries / physiology
Chemokine CCL2 / genetics
Endothelial Growth Factors / genetics*
Gene Expression / physiology
Intercellular Signaling Peptides and Proteins / genetics*
Ligation
Lymphokines / genetics*
Male
Membrane Glycoproteins / genetics
Muscle, Skeletal / blood supply*,  physiology*
Neovascularization, Physiologic / physiology
Nitric Oxide Synthase / genetics
Nitric Oxide Synthase Type III
Physical Conditioning, Animal / physiology*
Physical Exertion / physiology*
RNA, Messenger / analysis
Rats
Rats, Sprague-Dawley
Receptor Protein-Tyrosine Kinases / genetics
Receptor, TIE-2
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor Receptor-1 / genetics
Vascular Endothelial Growth Factor Receptor-2 / genetics
Vascular Endothelial Growth Factors
Grant Support
ID/Acronym/Agency:
F32 HL 10406/HL/NHLBI NIH HHS; F32 HL 10485/HL/NHLBI NIH HHS; HL 37387/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Angiogenesis Inducing Agents; 0/Angiopoietin-1; 0/Angiopoietin-2; 0/Angpt1 protein, rat; 0/Chemokine CCL2; 0/Endothelial Growth Factors; 0/Intercellular Signaling Peptides and Proteins; 0/Lymphokines; 0/Membrane Glycoproteins; 0/RNA, Messenger; 0/Vascular Endothelial Growth Factor A; 0/Vascular Endothelial Growth Factors; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 1.14.13.39/Nos3 protein, rat; EC 2.7.10.1/Receptor Protein-Tyrosine Kinases; EC 2.7.10.1/Receptor, TIE-2; EC 2.7.10.1/Vascular Endothelial Growth Factor Receptor-1; EC 2.7.10.1/Vascular Endothelial Growth Factor Receptor-2

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