|Angiogenic growth factor expression in rat skeletal muscle in response to exercise training.|
|PMID: 12543634 Owner: NLM Status: MEDLINE|
|Angiogenesis occurs in skeletal muscle in response to exercise training. To gain insight into the regulation of this process, we evaluated the mRNA expression of factors implicated in angiogenesis over the course of a training program. We studied sedentary control (n = 17) rats and both sedentary (n = 18) and exercise-trained (n = 48) rats with bilateral femoral artery ligation. Training consisted of treadmill exercise (4 times/day, 1-24 days). Basal mRNA expression in sedentary control muscle was inversely related to muscle vascularity. Angiogenesis was histologically evident in trained white gastrocnemius muscle by day 12. Training produced initial three- to sixfold increases in VEGF, VEGF receptors (KDR and Flt), the angiopoietin receptor (Tie-2), and endothelial nitric oxide synthase mRNA, which dissipated before the increase in capillarity, and a substantial (30- to 50-fold) but transient upregulation of monocyte chemoattractant protein 1 mRNA. These results emphasize the importance of early events in regulating angiogenesis. However, we observed a sustained elevation of the angiopoietin 2-to-angiopoietin 1 ratio, suggesting continued vascular destabilization. The response to exercise was (in general) tempered in high-oxidative muscles. These findings place importance on cellular events coupled to the onset of angiogenesis.|
|Pamela G Lloyd; Barry M Prior; Hsiao T Yang; Ronald L Terjung|
Related Documents :
|15932964 - Effect of carbohydrate ingestion on exercise-induced alterations in metabolic gene expr...
14959564 - Expression of emerin and lamins in muscle of patients with different forms of emery-dre...
4031774 - Malleability of the system in overcoming limitations: functional elements.
9578384 - Exercise-induced changes in protein metabolism.
22890714 - Lifelong physical activity prevents an age-related reduction in arterial and skeletal m...
22162524 - Training-induced adaptation in purine metabolism in high-level sprinters vs. triathletes.
|Type: Journal Article; Research Support, U.S. Gov't, P.H.S. Date: 2003-01-23|
|Title: American journal of physiology. Heart and circulatory physiology Volume: 284 ISSN: 0363-6135 ISO Abbreviation: Am. J. Physiol. Heart Circ. Physiol. Publication Date: 2003 May|
|Created Date: 2003-04-07 Completed Date: 2003-05-15 Revised Date: 2011-08-03|
Medline Journal Info:
|Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: United States|
|Languages: eng Pagination: H1668-78 Citation Subset: IM|
|Department of Biomedical Sciences, College of Veterinary Medicine, University of Missouri, 1600 E. Rollins Road, Columbia, MO 65211, USA.|
|APA/MLA Format Download EndNote Download BibTex|
Angiogenesis Inducing Agents
Capillaries / physiology
Chemokine CCL2 / genetics
Endothelial Growth Factors / genetics*
Gene Expression / physiology
Intercellular Signaling Peptides and Proteins / genetics*
Lymphokines / genetics*
Membrane Glycoproteins / genetics
Muscle, Skeletal / blood supply*, physiology*
Neovascularization, Physiologic / physiology
Nitric Oxide Synthase / genetics
Nitric Oxide Synthase Type III
Physical Conditioning, Animal / physiology*
Physical Exertion / physiology*
RNA, Messenger / analysis
Receptor Protein-Tyrosine Kinases / genetics
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor Receptor-1 / genetics
Vascular Endothelial Growth Factor Receptor-2 / genetics
Vascular Endothelial Growth Factors
|F32 HL 10406/HL/NHLBI NIH HHS; F32 HL 10485/HL/NHLBI NIH HHS; HL 37387/HL/NHLBI NIH HHS|
|0/Angiogenesis Inducing Agents; 0/Angiopoietin-1; 0/Angiopoietin-2; 0/Angpt1 protein, rat; 0/Chemokine CCL2; 0/Endothelial Growth Factors; 0/Intercellular Signaling Peptides and Proteins; 0/Lymphokines; 0/Membrane Glycoproteins; 0/RNA, Messenger; 0/Vascular Endothelial Growth Factor A; 0/Vascular Endothelial Growth Factors; EC 220.127.116.11/Nitric Oxide Synthase; EC 18.104.22.168/Nitric Oxide Synthase Type III; EC 22.214.171.124/Nos3 protein, rat; EC 126.96.36.199/Receptor Protein-Tyrosine Kinases; EC 188.8.131.52/Receptor, TIE-2; EC 184.108.40.206/Vascular Endothelial Growth Factor Receptor-1; EC 220.127.116.11/Vascular Endothelial Growth Factor Receptor-2|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Uniaxial strain upregulates matrix-degrading enzymes produced by human vascular smooth muscle cells.
Next Document: Role of kappa-opioid receptor activation in pharmacological preconditioning of swine.