Document Detail


Angiogenic activity of the recombinant hst-1 protein.
MedLine Citation:
PMID:  7520355     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The hst-1 transforming gene encodes a protein which belongs to the FGF family of growth factors. We showed previously that a human hst-1 protein produced in silkworm cells has in vitro mitogenic activity to vascular endothelial cells. Here we report effective synthesis of an unfused human hst-1 protein in E. coli and a potent in vivo angiogenic activity of this hst-1 protein by two in vivo assays for angiogenesis, chick chorioallantoic membrane assay and rat cornea assay. The NIH3T3 transformant transfected with the hst-1 gene appeared to develop a highly-vascularized tumor on nude mice. These data showed that the hst-1 protein has an angiogenic activity in vivo as well as in vitro.
Authors:
T Yoshida; K Ishimaru; H Sakamoto; J Yokota; S Hirohashi; K Igarashi; K Sudo; M Terada
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer letters     Volume:  83     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  1994 Aug 
Date Detail:
Created Date:  1994-09-22     Completed Date:  1994-09-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  261-8     Citation Subset:  IM    
Affiliation:
Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chick Embryo
Cornea / blood supply
Fibroblast Growth Factor 4
Fibroblast Growth Factors / pharmacology*
Mice
Mice, Nude
Neoplasms, Experimental / blood supply
Neovascularization, Pathologic*
Proto-Oncogene Proteins / pharmacology*
Rats
Recombinant Proteins
Chemical
Reg. No./Substance:
0/Fgf4 protein, mouse; 0/Fgf4 protein, rat; 0/Fibroblast Growth Factor 4; 0/Proto-Oncogene Proteins; 0/Recombinant Proteins; 62031-54-3/Fibroblast Growth Factors

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