| Angiogenesis stimulation in explanted hearts from patients pre-treated with intravenous prostaglandin E(1). | |
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MedLine Citation:
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PMID: 11295585 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Prostaglandin E(1) (PGE(1)) is a potent vasodilator and induces angiogenesis in animal tissues. Previous clinical studies demonstrated that PGE(1) improves hemodynamic parameters in patients with heart failure listed for heart transplantation (HTX). Therefore, we designed a retrospective immunohistochemistry study to investigate various markers of angiogenesis using hearts explanted from PGE(1)-treated patients with idiopathic dilated cardiomyopathy (IDCM). METHODS AND RESULTS: We investigated neovascularization in 18 hearts explanted from patients with IDCM: 9 patients received treatment with chronic infusions of PGE(1) for end-stage heart failure before HTX, whereas the remaining patients with IDCM did not receive PGE(1) and served as controls. We used immunoreactivity against CD34, von Willebrand factor (vWf), vascular endothelial growth factor (VEGF), and MIB-1 (Ki-67) to quantify angiogenesis, and used sirius red staining to determine the degree of fibrosis. Compared with the control group, PGE(1)-treated patients had significantly more CD34-, vWf- and MIB-1-positive cells in the sub-endocardium, myocardium and sub-epicardium (p < 0.01). The degree of fibrosis in the hearts of PGE(1)-treated patients was significantly lower than in control patients (p < 0.05), but we did not see any difference in the percentage of muscle mass. Finally, throughout the ventricles, we found significantly more VEGF-positive capillaries in the PGE(1) group (p < 0.0001). CONCLUSIONS: The data suggest that PGE(1) could be a potent inducer of angiogenesis and the angiogenic factor VEGF, and could cause reduced fibrosis in the failing human heart. |
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Authors:
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M R Mehrabi; C Ekmekcioglu; B Stanek; T Thalhammer; F Tamaddon; R Pacher; G E Steiner; T Wild; M Grimm; P G Spieckermann; G Mall; H D Glogar |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation Volume: 20 ISSN: 1053-2498 ISO Abbreviation: J. Heart Lung Transplant. Publication Date: 2001 Apr |
Date Detail:
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Created Date: 2001-04-11 Completed Date: 2001-07-05 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 9102703 Medline TA: J Heart Lung Transplant Country: United States |
Other Details:
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Languages: eng Pagination: 465-73 Citation Subset: IM |
Affiliation:
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Department of Cardiology, University of Vienna, Vienna, Austria. heartreza@hotmail.com |
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| MeSH Terms | |
Descriptor/Qualifier:
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Alprostadil
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pharmacology* Antigens, CD34 / drug effects, metabolism Antigens, Nuclear Endothelial Growth Factors / metabolism Female Fibrosis Heart Ventricles / drug effects Humans Immunohistochemistry Ki-67 Antigen Male Middle Aged Myocardium / cytology Neovascularization, Physiologic / drug effects* Nuclear Proteins / drug effects, metabolism Retrospective Studies Vasodilator Agents / pharmacology* von Willebrand Factor / drug effects, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD34; 0/Antigens, Nuclear; 0/Endothelial Growth Factors; 0/Ki-67 Antigen; 0/Nuclear Proteins; 0/Vasodilator Agents; 0/von Willebrand Factor; 745-65-3/Alprostadil |
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