| Angiogenesis in a delayed revascularization model is accelerated by angiogenic oligosaccharides of hyaluronan. | |
| | |
MedLine Citation:
|
PMID: 7543630 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: A delayed revascularisation model has been used to assess the angiogenic activity of hyaluronan fragments on impaired wound healing. EXPERIMENTAL DESIGN: Revascularisation of single, full thickness skin autografts in rats was delayed by subjecting isolated grafts to a sublethal cryoinjury (freeze injury) before implantation. Hyaluronan fragments were delivered to the grafts using slow release pellets (ethylene vinyl acetate copolymer). Rates of release were measured in vitro by ELISA. The angiogenic response to the application of 100 micrograms of low (1 to 4 kDa) molecular weight hyaluronan and 100 micrograms of medium (33 kDa) molecular weight hyaluronan was tested in separate groups of 15 rats and was compared with unstimulated cryoinjured controls (n = 40). The effect of low (1 to 4 kDa) molecular weight hyaluronan on uninjured grafts was also investigated. Return of graft blood flow was measured on anesthetised animals over a 10-day period using laser Doppler flowmetry and 133Xe clearance. Quantitative histologic assessment of the graft vasculature was performed on Days 3, 7, and 10 after implantation. RESULTS: The 1- to 4-kDa hyaluronan fragments increased blood flow (p < 0.001), as measured by both flow measuring techniques, and increased graft vessel growth, as assessed histologically at each of the three time points (p < 0.05). By contrast, the 33-kDa fragments had no such effect on graft blood flow or vessel growth. Low molecular weight hyaluronan had no effect on either graft blood flow or on vessel growth in uninjured skin grafts. CONCLUSIONS: The hypothesis that there may be physiologic regulation of angiogenesis by hyaluronan and its metabolites is supported by the results of these studies. The data provide further evidence of the utility of the cryoinjured graft model for the study of in vivo angiogenesis. |
| | |
Authors:
|
V C Lees; T P Fan; D C West |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Laboratory investigation; a journal of technical methods and pathology Volume: 73 ISSN: 0023-6837 ISO Abbreviation: Lab. Invest. Publication Date: 1995 Aug |
Date Detail:
|
Created Date: 1995-09-14 Completed Date: 1995-09-14 Revised Date: 2009-09-29 |
Medline Journal Info:
|
Nlm Unique ID: 0376617 Medline TA: Lab Invest Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 259-66 Citation Subset: IM |
Affiliation:
|
Department of Pharmacology, University of Cambridge, England. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blood Flow Velocity Disease Models, Animal Hyaluronic Acid / chemistry*, metabolism, pharmacology* Male Neovascularization, Pathologic / chemically induced* Oligosaccharides / analysis* Polyvinyls / metabolism Rats Rats, Wistar Skin Transplantation / pathology Wound Healing / physiology |
| Grant Support | |
ID/Acronym/Agency:
|
//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
|
0/Oligosaccharides; 0/Polyvinyls; 24937-78-8/ethylenevinylacetate copolymer; 9004-61-9/Hyaluronic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Virus-induced increases in bronchiolar mast cells in Brown Norway rats are associated with both loca...
Next Document: Effect of growth factors on cell proliferation and epithelialization in human skin.