| Aneuploidogenic and clastogenic potential of the mycotoxins citrinin and patulin. | |
| | |
MedLine Citation:
|
PMID: 9683194 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The aneuploidogenic and clastogenic potentials of the mycotoxins citrinin (CIT) and patulin (PAT) were studied by determining inhibition of microtubule assembly under cell-free conditions and by measuring induction of mitotic arrest and micronuclei in cultured Chinese hamster V79 cells. Both CIT and PAT inhibited cell-free microtubule polymerization in a concentration-dependent manner. PAT, but not CIT, bound covalently to reactive thiol groups of microtubule proteins. At concentrations without gross cytotoxicity, mitotic arrest and CREST-positive micronuclei, i.e. micronuclei containing whole chromosomes/chromatids, were induced by CIT and PAT in V79 cells. The time course of micronucleus induction and positive CREST staining indicate the aneuploidogenic potential of CIT and PAT. CREST-negative micronuclei, i.e. micronuclei containing acentric chromosomal fragments, were induced by PAT but not by CIT, implying a clastogenic potential of PAT. The aneuploidogenic and clastogenic potential of PAT and the aneuploidogenic potential of CIT may well contribute to the putative carcinogenicity of these mycotoxins in long-term animal studies. |
| | |
Authors:
|
E Pfeiffer; K Gross; M Metzler |
Related Documents
:
|
15980204 - Isolation of a protein interacting with vfphot1a in guard cells of vicia faba. 2253844 - Microtubule dynamics. 1412704 - Microtubule motors: many new models off the assembly line. 19818614 - A rop gtpase signaling pathway controls cortical microtubule ordering and cell expansio... 12576544 - Disruption of 3d tissue integrity facilitates adenovirus infection by deregulating the ... 9370964 - Electrophysiological basis of human fallopian tubal fluid formation. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Carcinogenesis Volume: 19 ISSN: 0143-3334 ISO Abbreviation: Carcinogenesis Publication Date: 1998 Jul |
Date Detail:
|
Created Date: 1998-08-06 Completed Date: 1998-08-06 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8008055 Medline TA: Carcinogenesis Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 1313-8 Citation Subset: IM |
Affiliation:
|
Institute of Food Chemistry, University of Karlsruhe, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aneuploidy* Animals Anti-Bacterial Agents / toxicity* Cattle Cells, Cultured Chromosome Aberrations Chromosomes / drug effects Citrinin / toxicity* Cricetinae Cricetulus Fibroblasts / cytology, drug effects, ultrastructure Male Metaphase / drug effects Micronuclei, Chromosome-Defective / drug effects Microtubules / drug effects Mutagens / toxicity* Patulin / toxicity* Sulfhydryl Compounds / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Anti-Bacterial Agents; 0/Mutagens; 0/Sulfhydryl Compounds; 149-29-1/Patulin; 518-75-2/Citrinin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Induction of nuclear catechol-O-methyltransferase by estrogens in hamster kidney: implications for e...
Next Document: X-radiation induces 8-hydroxy-2'-deoxyguanosine formation in vivo in rat mammary gland DNA.