Document Detail

Aneuploid mosaicism in the developing and adult cerebellar cortex.
MedLine Citation:
PMID:  18273885     Owner:  NLM     Status:  MEDLINE    
Neuroprogenitor cells (NPCs) in several telencephalic proliferative regions of the mammalian brain, including the embryonic cerebral cortex and postnatal subventricular zone (SVZ), display cell division "defects" in normal cells that result in aneuploid adult progeny. Here, we identify the developing cerebellum as a major, nontelencephalic proliferative region of the vertebrate central nervous system (CNS) that also produces aneuploid NPCs and nonmitotic cells. Mitotic NPCs assessed by metaphase chromosome analyses revealed that 15.3% and 20.8% of cerebellar NPCs are aneuploid at P0 and P7, respectively. By using immunofluorescent analysis of cerebellar NPCs, we show that chromosome segregation defects contribute to the generation of cells with an aneuploid genomic complement. Nonmitotic cells were assessed by fluorescence-activated cell sorting (FACS) coupled with fluorescence in situ hybridization (FISH), which revealed neuronal and nonneuronal aneuploid populations in both the adult mouse and human cerebellum. Taken together, these results demonstrate that the prevalence of neural aneuploidy includes nontelencephalic portions of the neuraxis and suggest that the generation and maintenance of aneuploid cells is a widespread, if not universal, property of central nervous system development and organization.
Jurjen W Westra; Suzanne E Peterson; Yun C Yung; Tetsuji Mutoh; Serena Barral; Jerold Chun
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of comparative neurology     Volume:  507     ISSN:  1096-9861     ISO Abbreviation:  J. Comp. Neurol.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-02-26     Completed Date:  2008-03-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0406041     Medline TA:  J Comp Neurol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1944-51     Citation Subset:  IM    
Copyright Information:
(c) 2008 Wiley-Liss, Inc.
Helen L. Dorris Child and Adolescent Neuropsychiatric Disorder Institute, The Scripps Research Institute, La Jolla, California 92037, USA.
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MeSH Terms
Aging / genetics
Animals, Newborn
Cell Differentiation / genetics
Cell Proliferation
Cerebellar Cortex / cytology,  growth & development*
Chromosome Segregation / genetics
Chromosomes / genetics
Flow Cytometry
Fluorescent Antibody Technique
In Situ Hybridization, Fluorescence
Metaphase / genetics
Mice, Inbred BALB C
Mitosis / genetics
Neurons / cytology,  physiology*
Stem Cells / cytology,  physiology*
Grant Support

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