| Androstenedione induces abnormalities in morphology and function of developing oocytes, which impairs oocyte meiotic competence. | |
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MedLine Citation:
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PMID: 22289288 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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OBJECTIVE: To obtain insight into the effects of androstenedione on ovarian folliculogenesis and oogenesis. DESIGN: Experimental study. SETTING: St. Marianna University School of Medicine. ANIMAL(S): Prepubertal (14-day-old) BDF1 female mice. INTERVENTION(S): Early secondary follicles were isolated from the ovaries and were cultured individually in vitro with or without androstenedione (10(-11) to 10(-5) M) for 12 days. Thereafter, the follicles were treated with hCG and epidermal growth factor (EGF). MAIN OUTCOME MEASURE(S): Diameters and morphology of follicles and oocytes; E(2) and P secretion; and chromatin configuration and expression of growth differentiation factor 9 (GDF9) in oocytes were examined. RESULT(S): Early secondary follicles developed to the preovulatory stage. Androstenedione treatments increased the follicle diameters, reduced survival rates of follicles, and promoted the formation of follicles with abnormal morphology, including misshapen oocyte. The secretion of E(2) and P was significantly higher in androstenedione-exposed follicles. Androstenedione prevented the alteration in chromatin configuration and reduced oocyte GDF9 expression. When follicles cultured with androstenedione were treated with hCG and EGF, the first polar body exclusion, chromosome alignment on metaphase plate, and spindle assembly were inhibited in the oocytes. CONCLUSION(S): These results demonstrate that excess androgen induces abnormalities in the morphology and function of developing oocytes, which impairs oocyte meiotic competence. |
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Authors:
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Wataru Tarumi; Sanae Tsukamoto; Yuki Okutsu; Noriyuki Takahashi; Toshitaka Horiuchi; Masanori T Itoh; Bunpei Ishizuka |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Fertility and sterility Volume: 97 ISSN: 1556-5653 ISO Abbreviation: Fertil. Steril. Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-01-31 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: United States |
Other Details:
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Languages: eng Pagination: 469-76 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Obstetrics and Gynecology, St. Marianna University School of Medicine, Kawasaki, Japan; Department of Anatomy, St. Marianna University School of Medicine, Kawasaki, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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