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Andrographolide protects against lipopolysaccharide-induced vascular hyporeactivity by suppressing the expression of inducible nitric oxide in periaortic adipose.
MedLine Citation:
PMID:  23575260     Owner:  NLM     Status:  Publisher    
This study investigated the role of perivascular adipose tissue (PVAT) in the beneficial effects of andrographolide on vascular reactivity in endotoxaemic rats. After being challenged by lipopolysaccharide (LPS, 4 mg/kg, i.p.), the rats were treated with andrographolide (1 mg/kg, i.p.). The response to phenylephrine of aortic rings with or without PVAT was recorded. Vascular relaxing effect of PVAT was determined by bioassay experiments. Inducible nitric oxide synthase (iNOS) in aortic PVAT was tested by western-blot, immunofluorescence and qPCR. LPS injection lowered the contraction force induced by phenylephrine in aortic rings with or without PVAT and andrographolide treatment reversed these effects. In bioassay experiments, transferring bathing solution incubated with a PVAT+ ring to a PVAT- ring induced relaxation in the recipient. This relaxing effect of PVAT from endotoxaemic rats was more potent than the rats treated with vehicles. Andrographolide treatment decreased the relaxing effect of PVAT in endotoxaemic rats. The levels of iNOS protein and mRNA in PVAT were significantly higher in endotoxaemic rats than the rats treated with vehicles. Andrographolide treatment decreased PVAT iNOS protein and mRNA levels in endotoxaemic rats. Our results suggest andrographolide restores vascular reactivity in endotoxaemic rats by down-regulation of iNOS in PVAT.
Liu Hai-Mei; Huang Song-Yin; Li Run-Mei; Xu Xiao-Huang; Zhou Le-Quan; Lai Xiao-Ping; Xu Jin-Wen
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-4-9
Journal Detail:
Title:  Journal of cardiovascular pharmacology     Volume:  -     ISSN:  1533-4023     ISO Abbreviation:  J. Cardiovasc. Pharmacol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-4-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7902492     Medline TA:  J Cardiovasc Pharmacol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
1Department of physiology, Basic medical college, Guangzhou University of Chinese Medicine, University Town, Guangzhou 510006, China. 2Department of Laboratory, Sun Yat-Sen Memorial Hospital, SunYat-Sen University, Guangzhou 510120, China. 3School of Chinese Pharmaceutical Science, Guangzhou University of Chinese Medicine, University Town, Guangzhou 510006, China.
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