Document Detail


Androgens modulate structure and function of the suprachiasmatic nucleus brain clock.
MedLine Citation:
PMID:  21363939     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Gonadal hormones can modulate circadian rhythms in rodents and humans, and androgen receptors are highly localized within the core region of the mouse suprachiasmatic nucleus (SCN) brain clock. Although androgens are known to modulate neural plasticity in other CNS compartments, the role of androgens and their receptors on plasticity in the SCN is unexplored. In the present study, we ask whether androgens influence the structure and function of the mouse SCN by examining the effects of gonadectomy (GDX) on the structure of the SCN circuit and its responses to light, including induction of clock genes and behavioral phase shifting. We found that after GDX, glial fibrillary acidic protein increased with concomitant decreases in the expression of the synaptic proteins synaptophysin and postsynaptic density 95. We also found that GDX exerts effects on the molecular and behavioral responses to light that are phase dependent. In late night [circadian time (CT)21], GDX increased light-induced mPer1 but not mPer2 expression compared with intact (INT) controls. In contrast, in early night (CT13.5), GDX decreased light induced mPer2 but had no effect on mPer1. At CT13.5, GDX animals also showed larger phase delays than did INT. Treatment of GDX animals with the nonaromatizable androgen dihydrotestosterone restored glial fibrillary acidic protein, postsynaptic density 95, and synaptophysin in the SCN and reinstated the INT pattern of molecular and behavioral responses to light. Together, the results reveal a role for androgens in regulating circuitry in the mouse SCN, with functional consequences for clock gene expression and behavioral responses to photic phase resetting stimuli.
Authors:
Ilia N Karatsoreos; Matthew P Butler; Joseph Lesauter; Rae Silver
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-03-01
Journal Detail:
Title:  Endocrinology     Volume:  152     ISSN:  1945-7170     ISO Abbreviation:  Endocrinology     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-04-22     Completed Date:  2011-07-22     Revised Date:  2012-05-01    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1970-8     Citation Subset:  AIM; IM    
Affiliation:
Department of Psychology, Columbia University, New York, New York 10027, USA.
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MeSH Terms
Descriptor/Qualifier:
Androgens / pharmacology*
Animals
Biological Clocks / drug effects*,  genetics,  physiology
Blotting, Western
Brain / drug effects,  metabolism,  radiation effects
Circadian Rhythm
Dihydrotestosterone / pharmacology*
Gene Expression / drug effects,  radiation effects
Glial Fibrillary Acidic Protein / metabolism
Guanylate Kinase
In Situ Hybridization
Intracellular Signaling Peptides and Proteins / metabolism
Light
Male
Membrane Proteins / metabolism
Mice
Mice, Inbred C57BL
Microscopy, Confocal
Motor Activity / drug effects,  radiation effects
Orchiectomy
Period Circadian Proteins / genetics
Suprachiasmatic Nucleus / drug effects*,  metabolism
Synaptophysin / metabolism
Time Factors
Grant Support
ID/Acronym/Agency:
NS37919/NS/NINDS NIH HHS; T32DK07328/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Androgens; 0/Glial Fibrillary Acidic Protein; 0/Intracellular Signaling Peptides and Proteins; 0/Membrane Proteins; 0/Per1 protein, mouse; 0/Per2 protein, mouse; 0/Period Circadian Proteins; 0/Synaptophysin; 521-18-6/Dihydrotestosterone; EC 2.7.4.8/Dlgh4 protein, mouse; EC 2.7.4.8/Guanylate Kinase
Comments/Corrections
Comment In:
Endocrinology. 2011 May;152(5):1727-30   [PMID:  21511983 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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