| Androgen receptor in genital tubercle of rabbit fetuses and newborns. Ontogeny and properties. | |
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MedLine Citation:
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PMID: 3840856 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In newborn rabbits of both sexes, an androgen receptor was characterized in the genital tubercle. Homogenates exhibited high affinity (Kd was about 0.4 nM) and saturable binding of [3H]methyltrienolone. The half-life of the [3H]5 alpha-dihydrotestosterone-androgen receptor complex was 72 h at 4 degrees C. The receptor was inactivated by heat and pronase and the binding was specific for potent androgens. Sucrose gradient analysis revealed a 8-9 S [3H]methyltrienolone binding protein in cytosols from both sexes. Androgen binding, in the homogenate, was detected as soon as day 18 of gestation in both sexes and the number of binding sites increased until birth. During sexual organogenesis and at birth there were no major differences between males and females in the amount or affinity of androgen binding. Specific androgen binding was also detected in sexual ducts of male and female newborns. |
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Authors:
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G Veyssiere; M Berger; C Jean-Faucher; M de Turckheim; C Jean |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of steroid biochemistry Volume: 23 ISSN: 0022-4731 ISO Abbreviation: J. Steroid Biochem. Publication Date: 1985 Oct |
Date Detail:
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Created Date: 1986-01-14 Completed Date: 1986-01-14 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0260125 Medline TA: J Steroid Biochem Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 399-404 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn / metabolism Centrifugation, Density Gradient Female Fetus / analysis* Genitalia, Female / analysis*, embryology Genitalia, Male / analysis*, embryology Hot Temperature Male Pregnancy Pronase / pharmacology Rabbits Receptors, Androgen / analysis* Sex Differentiation Sex Factors Tritium / diagnostic use |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Androgen; 10028-17-8/Tritium; EC 3.4.24.-/Pronase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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