Document Detail


Androgen cytosol binding in exercise-induced sparing of muscle atrophy.
MedLine Citation:
PMID:  6333826     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was undertaken to determine whether the exercise-induced sparing of glucocorticoid-induced muscle atrophy is related to increased androgen cytosol binding. Female rats were divided into a sedentary or an exercise group that was trained by treadmill running 100 min/day for 13-15 wk. During the last 12 days of training, each of these groups was further subdivided into groups that received daily subcutaneous injections of cortisone acetate (CA) (100 mg/kg body wt) or the vehicle 1% carboxymethyl cellulose. Exercise prevented 30-40% of the weight loss due to CA treatment in gastrocnemius and plantaris muscles. Scatchard analyses of specific binding of [3H]methyltrienolone (R1881), a synthetic androgen that binds to androgen receptors, were nonlinear in muscles from vehicle-treated sedentary and trained rats and were resolved by a two-component binding model. The lower affinity component, which was attributed to a glucocorticoid receptor, disappeared in muscles of glucocorticoid-treated animals as evidenced by linear Scatchard plots. Receptor concentrations of the androgenic component of [3H]methyltrienolone binding were similar in gastrocnemius and plantaris muscles in all treatment groups. In binding specificity studies of gastrocnemius muscles, the relatively high competition by various glucocorticoids and progesterone for [3H]methyltrienolone binding in the vehicle-treated groups was reduced by CA treatment. The lack of change in androgen cytosol receptor levels suggests that this is not a mechanism by which exercise protects against glucocorticoid-induced muscle atrophy.
Authors:
R C Hickson; T T Kurowski; J A Capaccio; R T Chatterton
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  247     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1984 Nov 
Date Detail:
Created Date:  1984-12-19     Completed Date:  1984-12-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  E597-603     Citation Subset:  IM; S    
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MeSH Terms
Descriptor/Qualifier:
Androgens / metabolism*
Animals
Body Weight
Cortisone / analogs & derivatives,  toxicity
Cytosol / metabolism
Estrenes / metabolism
Female
Metribolone
Muscular Atrophy / chemically induced,  metabolism*
Physical Exertion*
Rats
Rats, Inbred Strains
Receptors, Androgen / metabolism*
Receptors, Glucocorticoid / metabolism
Receptors, Steroid / metabolism*
Testosterone Congeners / metabolism
Grant Support
ID/Acronym/Agency:
AM-26408/AM/NIADDK NIH HHS; K04-AM-01100/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Androgens; 0/Estrenes; 0/Receptors, Androgen; 0/Receptors, Glucocorticoid; 0/Receptors, Steroid; 0/Testosterone Congeners; 50-04-4/cortisone acetate; 53-06-5/Cortisone; 965-93-5/Metribolone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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