Document Detail

'Ancient' DNA in the resting egg bank of a microcrustacean can serve as a palaeolimnological database.
MedLine Citation:
PMID:  11839197     Owner:  NLM     Status:  MEDLINE    
Recent work on the diapausing egg banks of zooplankton, such as Daphnia (Crustacea: Anomopoda), indicates that these eggs can remain viable for decades while, theoretically, DNA can remain intact for even longer periods (i.e. centuries or millennia). We isolated diapausing eggs of Daphnia from a 30 m long sediment core taken from a hypereutrophic, northern German lake (Belauer See), with some eggs found in dated core material as old as 4500 years. Using microsatellite markers, we analysed the genetic structure of the resting eggs dated as old as ca. 200 years, and found that, although levels of heterozygosity remained remarkably stable, significant genetic differentiation (Nei's D = 0.36; F(ST) = 0.15) between recent and 'ancient' resting eggs (including allele frequency shifts and private alleles) was detected. These shifts represent either species-level changes in this complex (i.e. species-specific characters of ephippia are not always robust), or intraspecific shifts in genetic variation, or a combination of both. This study demonstrates that the egg banks of aquatic zooplankton can serve as repositories of both genetic (intrapopulational) and ecological (interspecific) information. The use of molecular markers, such as microsatellites, on diapausing egg/seed banks may open new avenues of enquiry related to tracking the long-term genetic (and/or species) shifts that are associated with long-term environmental changes.
Petra A Limburg; Lawrence J Weider
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proceedings. Biological sciences / The Royal Society     Volume:  269     ISSN:  0962-8452     ISO Abbreviation:  Proc. Biol. Sci.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-02-12     Completed Date:  2002-06-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  101245157     Medline TA:  Proc Biol Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  281-7     Citation Subset:  IM    
Max Planck Institute for Limnology, August-Thienemann-Strasse 2, 24306 Plön, Germany.
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MeSH Terms
Crustacea / cytology*,  genetics*
DNA / genetics,  isolation & purification*
Databases, Nucleic Acid
Fresh Water / parasitology
Genetics, Population*
Microsatellite Repeats / genetics
Mutation / genetics
Ovum / metabolism*
Time Factors
Zooplankton / cytology*,  genetics*
Reg. No./Substance:

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