Document Detail

Anatomical location of arterial and venous lines significantly affects motor performance in rats.
MedLine Citation:
PMID:  22943533     Owner:  NLM     Status:  In-Data-Review    
Several motor-function scales have been developed to assess neurological function in animal models of stroke, subarachnoid hemorrhage and closed head injury. We hypothesize that the location of arterial and venous catheters, even in the absence of brain injury, may impact rats' motor performance. Our study examined the effect of catheter location, rate of infection and the time required for catheter placement. We further describe an original technique of tail artery cannulation without exposure of the artery. Sixty-one rats were anesthetized and randomly assigned to one of seven groups, including no catheter, tail artery or artery + vein catheters, or femoral artery or artery + vein catheters. A neurological severity score (NSS) was determined at 1 h, 24 h and 48 h after surgical preparation or catheter placement. NSS at 1 h after placement of unilateral and bilateral femoral catheters was higher than the NSS observed at 1 h after placement of tail arterial and venous catheters (P < 0.01). The NSS also was higher at 24 h in the bilateral femoral catheter groups as compared with the tail catheter groups (P < 0.05). There were no differences in the NSS observed between the groups that had tail catheters and the sham group at 1 h, 24 h or 48 h. Infection rate at the site of catheter placement and the time required for catheter placement was also higher in the femoral catheter groups (P < 0.001). Thus, we propose that the line location may bias a study's results and lead to deceptive interpretations of neurological assessment following rat head injury. Compared to femoral vessels, tail blood vessels are preferable locations for lines placement.
Sharon Ohayon; Shaun E Gruenbaum; Alan A Artru; Matthew Boyko; Benjamin F Gruenbaum; Michael Dubilet; Akiva Leibowitz; Yoram Shapira; Vivian I Teichberg; Alexander Zlotnik
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Publication Detail:
Type:  Journal Article     Date:  2012-04-25
Journal Detail:
Title:  Animal science journal = Nihon chikusan Gakkaihō     Volume:  83     ISSN:  1740-0929     ISO Abbreviation:  Anim. Sci. J.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100956805     Medline TA:  Anim Sci J     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  656-62     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors. Animal Science Journal © 2012 Japanese Society of Animal Science.
Department of Anesthesiology and Critical Care, Soroka Medical Center, Ben Gurion University of the Negev, Beer Sheva Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel Department of Anesthesiology, Yale University School of Medicine, New Haven, Connecticut Department of Anesthesiology, University of Washington, Seattle, Washington, USA.
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