Document Detail


Anatomical and functional recovery by embryonic stem cell-derived neural tissue of a mouse model of brain damage.
MedLine Citation:
PMID:  15050446     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have treated undifferentiated mouse embryonic stem (ES) cells with all-trans retinoic acid (RA) to induce differentiation in vitro into neuron-like cells with good cell viability for use as a graft. Furthermore, we asked whether the RA-induced neuron-like cells restored neurological dysfunction. To this end, the cells were transplanted into right hemiplegia model of mice, developed by a cryogenic injury of motor cortex. Motor function of the recipients was gradually improved, whereas little improvement was observed in control mice. The lesion showed clustering of mature and almost mature neuron-like cells in mice transplanted with the RA-treated cells. The grafted cells had synaptic vesicles. This finding may suggest their maturation and synaptic connection in the recipient brain. Even though further study is necessary to elucidate molecular and cellular mechanisms responsible for the functional recovery, we consider that the ES cells may have advantage for use as a donor source in various neurological disorders including motor dysfunction.
Authors:
Shunmei Chiba; Ritsuko Ikeda; Manae S Kurokawa; Hideshi Yoshikawa; Mitsuhiro Takeno; Hiroko Nagafuchi; Mamoru Tadokoro; Hiroaki Sekino; Takuo Hashimoto; Noboru Suzuki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the neurological sciences     Volume:  219     ISSN:  0022-510X     ISO Abbreviation:  J. Neurol. Sci.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-03-30     Completed Date:  2004-06-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375403     Medline TA:  J Neurol Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  107-17     Citation Subset:  IM    
Affiliation:
Department of Immunology, St. Marianna University School of Medicine, Kawasaki, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents / pharmacology
Brain Injuries / pathology,  therapy*
Brain Tissue Transplantation*
Cell Differentiation / drug effects
Endothelium, Vascular / cytology
Hemiplegia / pathology,  therapy
Mice
Mice, Inbred C57BL
Motor Cortex / injuries,  pathology
Movement
Neurons / cytology,  transplantation*
Recovery of Function
Stem Cell Transplantation*
Stem Cells / cytology,  drug effects
Tretinoin / pharmacology
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 302-79-4/Tretinoin

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