Document Detail


Analytical studies on the chiral separation and simultaneous determination of pantothenic acid and hopantenic acid enantiomers in rat plasma by gas chromatography-mass fragmentography.
MedLine Citation:
PMID:  1860905     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The chiral separation and simultaneous determination of D- and L-pantothenic acids and D- and L-hopantenic acids in rat plasma using gas chromatography-mass fragmentography are described. The method is based on deproteinization by ion-exchange resin, extraction with ethyl acetate under acidic conditions, and derivatization to form several interesting compounds. After methyl esterification for carboxylic acid of D- and L-pantothenic acids, D- and L-hopantenic acids could be derivatized to trifluoroacetate, cyclic sulphite, and cyclic n-butylboronate for hydroxy groups. D- and L-forms of these derivatives were completely separated by mass fragmentographic technique with quasi-molecular ions. Calcium salt of D-5-[(2,4-dihydroxy-3,3-dimethyl-1-oxobutyl)amino]pentanoic acid was used as an internal standard for the determination of DL-pantothenic acids and DL-hopantenic acids. The detection limits of DL-pantothenic acids and DL-hopantenic acids in this method were 5 and 12 ng, respectively. This method could be applied to the study of plasma levels of D-, L-pantothenic acids and D-, L-hopantenic acids in rat.
Authors:
K Banno; S Horimoto; M Matsuoka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  564     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1991 Mar 
Date Detail:
Created Date:  1991-08-30     Completed Date:  1991-08-30     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  1-10     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Gas Chromatography-Mass Spectrometry / methods*,  standards
Pantothenic Acid / analogs & derivatives*,  blood*,  isolation & purification
Rats
Stereoisomerism
gamma-Aminobutyric Acid / analogs & derivatives*,  blood,  isolation & purification
Chemical
Reg. No./Substance:
1977-33-9/pantogab; 19F5HK2737/Pantothenic Acid; 56-12-2/gamma-Aminobutyric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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