Document Detail

Analytical separation of tea catechins and food-related polyphenols by high-speed counter-current chromatography.
MedLine Citation:
PMID:  16239007     Owner:  NLM     Status:  MEDLINE    
High-speed counter-current chromatography (HSCCC) using the type-J coil planet centrifuge was applied to compositional analysis of tea catechins and separation of other food-related polyphenols. The HSCCC separation of nine different standard compounds and those from extracts of commercial tea leaves was performed with a two-phase solvent system composed of tert-butyl methyl ether-acetonitrile-0.1% aqueous trifluoroacetic acid (TFA) (2:2:3, v/v/v) by eluting the upper organic phase at a flow rate of 2 ml/min. The main compounds in the extract of non-fermented green tea were found to be monomeric catechins, their galloylated esters and caffeine. In addition to these compounds, oxidized pigments, such as hydrophobic theaflavins (TFs) and polar thearubigins (TRs) were also separated and detected from the extracts of semi-fermented oolong tea and fermented black tea. Furthermore, several food-related polyphenols, such as condensed catechin oligomers (procyanidins), phenolic acids and flavonol glycosides were clearly separated under the same HSCCC condition. These separation profiles of HSCCC provide useful information about the hydrophobic diversity of these bioactive polyphenols present in various types of teas and food products.
Akio Yanagida; Atsushi Shoji; Yoichi Shibusawa; Heisaburo Shindo; Motoyuki Tagashira; Mitsuo Ikeda; Yoichiro Ito
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Publication Detail:
Type:  Journal Article     Date:  2005-10-18
Journal Detail:
Title:  Journal of chromatography. A     Volume:  1112     ISSN:  0021-9673     ISO Abbreviation:  J Chromatogr A     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-04-17     Completed Date:  2006-06-27     Revised Date:  2009-01-15    
Medline Journal Info:
Nlm Unique ID:  9318488     Medline TA:  J Chromatogr A     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  195-201     Citation Subset:  IM    
Division of Structural Biology and Analytical Science, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
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MeSH Terms
Biflavonoids / isolation & purification
Camellia sinensis / chemistry*
Catechin / analogs & derivatives*,  isolation & purification*
Countercurrent Distribution / methods*
Flavonoids / isolation & purification*
Phenols / isolation & purification*
Tea / chemistry
Reg. No./Substance:
0/Biflavonoids; 0/Flavonoids; 0/Phenols; 0/Tea; 0/polyphenols; 12698-96-3/thearubigin; 154-23-4/Catechin; 4670-05-7/theaflavin

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