Document Detail


Analytical problems with the determination of coenzyme Q(10) in biological samples.
MedLine Citation:
PMID:  23303649     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The article discusses analytical problems related to the determination of coenzyme Q10 in biological samples. The assaying of coenzyme Q10 in complex samples, such as plasma, tissues, or food items requires meticulous sample preparation prior to final quantification. The process typically consists of the following steps: deproteinization, extraction, and ultimately reduction of extract volumes. At times drying under a gentle stream of neutral gas is applied. In the case of solid samples, a careful homogenization is also required. Each step of the sample preparation process can be a source of analytical errors that may lead to inaccurate results. The main aim of this work is to point to sources of analytical errors in the preparation process and their relation to physicochemical properties of coenzyme Q10. The article also discusses ways of avoiding and reducing the errors. © 2012 BioFactors, 2013.
Authors:
Monika Joanna Turkowicz; Joanna Karpińska
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-10
Journal Detail:
Title:  BioFactors (Oxford, England)     Volume:  -     ISSN:  1872-8081     ISO Abbreviation:  Biofactors     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807441     Medline TA:  Biofactors     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 International Union of Biochemistry and Molecular Biology, Inc.
Affiliation:
Voivodship Sanitary-Epidemiological Station in Bialystok, Food Examination Unit, Legionowa 8, 15-099 Białystok. monika.turkowicz@wp.pl.
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