| Analytical performance and clinical utility of a bioassay for thyroid-stimulating immunoglobulins. | |
| | |
MedLine Citation:
|
PMID: 23355204 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
The analytical performance and the clinical utility of a thyrotropin receptor (TSHR)-stimulating immunoglobulin (TSI) bioassay were compared with those of a TSHR-binding inhibitory immunoglobulin (TBII) assay. Limits of detection (LoD) and quantitation (LoQ), assay cutoff, and the half-maximal effective concentration (EC(50)) were measured. Dilution analysis was performed in sera of hyperthyroid patients with Graves disease (GD) during antithyroid treatment (ATD). Titer was defined as the first dilution step at which measurement of TSI or TBII fell below the assay cutoff. The LoD, LoQ, cutoff, and EC(50) of the bioassay were 251-, 298-, 814-, and 827-fold lower than for the TBII assay. There were 22%, 42%, 23%, and 14% more positive samples in the TSI bioassay at dilutions of 1:3, 1:9, 1:27, and 1:81 (P < .0001), respectively. Responders to ATD demonstrated marked differences in titers compared with nonresponders. The bioassay detected lower levels of TSHR autoantibodies, and the dilution analysis provided similar predictive values of both assays in GD. |
| | |
Authors:
|
Johannes J Leschik; Tanja Diana; Paul D Olivo; Jochem König; Ulrike Krahn; Yunsheng Li; Michael Kanitz; George J Kahaly |
Related Documents
:
|
21121614 - Determination of six thyroid hormones in the brain and thyroid gland using isotope-dilu... 16104774 - Development of time-resolved chemiluminescence for the determination of antu in river w... 12735474 - Determination of carbohydrates as their 3-aminophthalhydrazide derivatives by capillary... 18969864 - Determination of iodide by detection of iodine using gas-diffusion flow injection and c... 15993664 - Merits of hplc-based method over spectrophotometric method for assessing the kinetics a... 9023544 - Ion exchange hplc microanalysis of chondroitin sulfate: quantitative derivatization of ... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: American journal of clinical pathology Volume: 139 ISSN: 1943-7722 ISO Abbreviation: Am. J. Clin. Pathol. Publication Date: 2013 Feb |
Date Detail:
|
Created Date: 2013-01-28 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0370470 Medline TA: Am J Clin Pathol Country: United States |
Other Details:
|
Languages: eng Pagination: 192-200 Citation Subset: AIM; IM |
Affiliation:
|
Dept of Medicine I, Gutenberg University Medical Center, Mainz 55101, Germany; Kahaly@ukmainz.de. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Facilitating the Laboratory Diagnosis of ?1-Antitrypsin Deficiency.
Next Document: Establishment of a CYP2C19 Genotyping Assay for Clinical Use.