Document Detail

Analytical performance and clinical utility of a bioassay for thyroid-stimulating immunoglobulins.
MedLine Citation:
PMID:  23355204     Owner:  NLM     Status:  In-Data-Review    
The analytical performance and the clinical utility of a thyrotropin receptor (TSHR)-stimulating immunoglobulin (TSI) bioassay were compared with those of a TSHR-binding inhibitory immunoglobulin (TBII) assay. Limits of detection (LoD) and quantitation (LoQ), assay cutoff, and the half-maximal effective concentration (EC(50)) were measured. Dilution analysis was performed in sera of hyperthyroid patients with Graves disease (GD) during antithyroid treatment (ATD). Titer was defined as the first dilution step at which measurement of TSI or TBII fell below the assay cutoff. The LoD, LoQ, cutoff, and EC(50) of the bioassay were 251-, 298-, 814-, and 827-fold lower than for the TBII assay. There were 22%, 42%, 23%, and 14% more positive samples in the TSI bioassay at dilutions of 1:3, 1:9, 1:27, and 1:81 (P < .0001), respectively. Responders to ATD demonstrated marked differences in titers compared with nonresponders. The bioassay detected lower levels of TSHR autoantibodies, and the dilution analysis provided similar predictive values of both assays in GD.
Johannes J Leschik; Tanja Diana; Paul D Olivo; Jochem König; Ulrike Krahn; Yunsheng Li; Michael Kanitz; George J Kahaly
Related Documents :
23248034 - Simultaneous determination of 15 multiresidue organophosphorous pesticides in camellia ...
18966554 - Head-space flow injection for the on-line determination of iodide in urine samples with...
24176344 - Effect of ozone on aflatoxins detoxification and nutritional quality of peanuts.
337894 - Rapid identification and quantitation of small numbers of microorganisms by a chemilumi...
15272604 - Mechanism of the decrease of tetrodotoxin activity in modified seawater medium.
17631104 - Determination of quinolone residues in shrimp using liquid chromatography with fluoresc...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of clinical pathology     Volume:  139     ISSN:  1943-7722     ISO Abbreviation:  Am. J. Clin. Pathol.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370470     Medline TA:  Am J Clin Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  192-200     Citation Subset:  AIM; IM    
Dept of Medicine I, Gutenberg University Medical Center, Mainz 55101, Germany;
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Facilitating the Laboratory Diagnosis of ?1-Antitrypsin Deficiency.
Next Document:  Establishment of a CYP2C19 Genotyping Assay for Clinical Use.