Document Detail

Analytical method of chondroitin/dermatan sulfates using high performance liquid chromatography/turbo ionspray ionization mass spectrometry: application to analyses of the tumor tissue sections on glass slides.
MedLine Citation:
PMID:  11507718     Owner:  NLM     Status:  MEDLINE    
We established a highly sensitive quantitative analytical method for chondroitin/dermatan sulfates by LC/MS method. By this method, the unsaturated disaccharides produced after the enzymatic digestion of chondroitin/dermatan sulfates can be determined in the amounts as low as 0.5 pmol levels. The use of tetrabutylammonium hydroxide as an ion-pair reagent for LC/MS allowed us to separate unsaturated 4-sulfated disaccharide and unsaturated 6-sulfated disaccharide. Furthermore, the peak areas of unsaturated disaccharides were increased almost 10 times by the postcolumn addition of acetonitrile. We applied this LC/MS method to the analyses of unsaturated disaccharides from chondroitin/dermatan sulfates in the tissues sections on glass slides, which were prepared from MethA tumor-bearing mice. This method brought about considerable reduction in the time distance from sample collection to preparation of analytical results.
T Oguma; H Toyoda; T Toida; T Imanari
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biomedical chromatography : BMC     Volume:  15     ISSN:  0269-3879     ISO Abbreviation:  Biomed. Chromatogr.     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-08-16     Completed Date:  2001-11-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8610241     Medline TA:  Biomed Chromatogr     Country:  England    
Other Details:
Languages:  eng     Pagination:  356-62     Citation Subset:  IM    
Copyright Information:
Copyright 2001 John Wiley & Sons, Ltd.
Faculty of Pharmaceutical Sciences, Chiba University, Inage, Chiba, Japan.
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MeSH Terms
Chondroitin Sulfates / analysis*
Chromatography, High Pressure Liquid / methods*
Dermatan Sulfate / analysis*
Mass Spectrometry / methods*
Neoplasms / chemistry*,  pathology
Reproducibility of Results
Reg. No./Substance:
24967-94-0/Dermatan Sulfate; 9007-28-7/Chondroitin Sulfates

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