Document Detail


Analytical detection of immunoglobulin heavy chain gene rearrangements in gastric lymphoid infiltrates by peak area analysis of the melting curve in the LightCycler System.
MedLine Citation:
PMID:  17591935     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Because it is difficult to differentiate gastric mucosa-associated lymphoid tissue (MALT) lymphoma from chronic gastritis in gastric lymphoid infiltrates, molecular detection of monoclonality through immunoglobulin heavy chain (IgH) gene rearrangements is commonly performed. However, heterogeneity in the performance and results obtained from IgH gene rearrangements has been reported. To improve the accuracy in the diagnosis of gastric lymphoid infiltrates, we developed an analytical approach based on one-peak area analysis of the melting curve in the LightCycler System. Using a training-testing approach, the likelihood ratio method was selected to find a discriminative function of 4.64 in the training set (10 gastric MALT lymphomas and 10 chronic gastritis cases). This discriminative function was validated in the testing set (five gastric MALT lymphomas, six abnormal lymphocytic infiltrates with subsequently demonstrated gastric MALT lymphomas, and six cases of chronic gastritis). All but one case of gastric MALT lymphoma, as well as abnormal lymphocytic infiltrates, clustered under 4.64, and all chronic gastritis cases clustered above 4.64. These results were validated by conventional electrophoreses confirming one or two sharp bands in cases of gastric MALT lymphomas and a smear of multiple bands in cases of chronic gastritis. Analytical detection of IgH gene rearrangement in gastric lymphoid infiltrates by one-peak area analysis correctly distinguishes gastric MALT lymphomas from chronic gastritis, even in cases with diagnosis of abnormal lymphocytic infiltrates.
Authors:
Eduardo Retamales; Luis Rodriguez; Leda Guzman; Francisco Aguayo; Mariana Palma; Claudia Backhouse; Jorge Argandona; Erick Riquelme; Alejandro Corvalan
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of molecular diagnostics : JMD     Volume:  9     ISSN:  1525-1578     ISO Abbreviation:  J Mol Diagn     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-26     Completed Date:  2007-09-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  100893612     Medline TA:  J Mol Diagn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  351-7     Citation Subset:  IM    
Affiliation:
Departamento Laboratorio de Salud, Instituto Salud Publica de Chile, Santiago, Chile.
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MeSH Terms
Descriptor/Qualifier:
Biopsy
Chronic Disease
Diagnosis, Differential
Gastritis / diagnosis*,  genetics,  immunology,  pathology*
Gene Rearrangement, B-Lymphocyte, Heavy Chain*
Genes, Immunoglobulin Heavy Chain*
Humans
Lymphocytes, Tumor-Infiltrating*
Lymphoma, B-Cell, Marginal Zone / diagnosis*,  genetics,  immunology
Nucleic Acid Denaturation
Polymerase Chain Reaction / instrumentation*,  methods
Transition Temperature*
Comments/Corrections

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