Document Detail

Analytical and clinical evaluation of a new heart-type fatty acid-binding protein automated assay.
MedLine Citation:
PMID:  17087655     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The accurate and rapid recognition of myocardial injury in patients presenting in the emergency department (ED) with chest pain continues to be a clinical challenge. Heart-type fatty acid-binding protein (H-FABP) appears to be one of the best candidates among the new early cardiac markers studied. METHODS: We evaluated the analytical characteristics of a new quantitative and fully automated H-FABP assay (Randox Laboratories Ltd., Crumlin, UK) and compared its clinical performance with respect to the myoglobin (Myo) assay (Dade Behring, Milan, Italy). A precision study was carried out by testing three levels of quality control (QC) material and two in-house pool (P) samples. To test the accuracy of H-FABP determinations in plasma (lithium-heparin) samples, H-FABP concentrations measured in a set of matched sera and plasma samples were compared. A total of 77 non-consecutive patients (51 males and 26 females; 62+/-16 years) who presented to the ED with chest pain suggesting myocardial ischemia were enrolled. The patients were classified into two groups (acute myocardial infarction, n=22; non-acute myocardial infarction, n=55) on the basis of the discharge diagnosis. RESULTS: The between-day imprecision for three levels of control material and serum pool samples was 6.26%-8.04% (range 2.32-44.03 microg/L) and 9.03%-12.63% (range 11.85-65.13 microg/L), respectively. In the serum vs. plasma study, bias was +0.178 (95% CI -0.033 to +0.389). The best cut-off and the associated diagnostic efficacy were 95 microg/L and 89.47% for Myo and 5.09 microg/L and 98.70% for H-FABP, respectively. CONCLUSIONS: H-FABP determination in patients with ischemic symptoms may be a more reliable early indication of cardiac damage than myoglobin.
Martina Zaninotto; Monica Maria Mion; Enrica Novello; Sara Altinier; Stefano Rocco; Luisa Cacciavillani; Martina Perazzolo Marra; Sabino Iliceto; Mario Plebani
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Clinical chemistry and laboratory medicine : CCLM / FESCC     Volume:  44     ISSN:  1434-6621     ISO Abbreviation:  Clin. Chem. Lab. Med.     Publication Date:  2006  
Date Detail:
Created Date:  2006-11-07     Completed Date:  2007-01-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9806306     Medline TA:  Clin Chem Lab Med     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1383-5     Citation Subset:  IM    
Department of Laboratory Medicine, University Hospital of Padova, Padova, Italy.
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MeSH Terms
Biological Markers / blood
Blood Chemical Analysis / instrumentation,  methods*
Enzyme-Linked Immunosorbent Assay / instrumentation,  methods
Fatty Acid-Binding Proteins / blood*
Middle Aged
Myocardial Infarction / blood,  diagnosis*
Myoglobin / blood
Protein Array Analysis / instrumentation,  methods
ROC Curve
Reproducibility of Results
Sensitivity and Specificity
Reg. No./Substance:
0/Biological Markers; 0/FABP3 protein, human; 0/Fatty Acid-Binding Proteins; 0/Myoglobin

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