Document Detail


Analytic performance of the iQ200 automated urine microscopy analyzer and comparison with manual counts using Fuchs-Rosenthal cell chambers.
MedLine Citation:
PMID:  15842056     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Automated instruments that can examine urine for cells and particles have reduced the need for labor-intensive manual microscopy. We evaluated the performance of the Iris iQ200 Automated Urine Microscopy Analyzer (Iris Diagnostics, Chatsworth, CA) and compared results with manual cell and particle counts using Fuchs-Rosenthal counting chambers. Within-run imprecision (coefficient of variation) of the iQ200 for urine samples ranged from 3.0% to 45% for RBC counts between 1,029 and 3 x 10(6) cells/L, 3.4% to 40% for WBC counts between 1,006 and 4 x 10(6) cells/L, and 8.9% to 35% for epithelial cell counts between 93 and 4 x 10(6) cells/L. Between-run imprecision was 3.3% at 1,017 x 10(6) cells/L and 19.2% at 28 x 10(6) cells/L. There was good agreement between the iQ200 and manual cell counts (r > or = 0.94); however; the iQ200 produced lower results based on slopes of 0.92 (RBC count), 0.81 (WBC count), and 0.94 (epithelial cell counts). The iQ200 has satisfactory performance and correlates well with manual cell counts. Most urine samples containing RBCs, WBCs, and epithelial cells can be reported without review of captured images.
Authors:
David T Wah; Porntip K Wises; Anthony W Butch
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of clinical pathology     Volume:  123     ISSN:  0002-9173     ISO Abbreviation:  Am. J. Clin. Pathol.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-04-21     Completed Date:  2005-05-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370470     Medline TA:  Am J Clin Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  290-6     Citation Subset:  AIM; IM    
Affiliation:
Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at the University of California Los Angeles, 90095-1732, USA.
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MeSH Terms
Descriptor/Qualifier:
Autoanalysis / instrumentation
Cell Count / instrumentation*,  methods
Humans
Microscopy*
Reproducibility of Results
Urinalysis / instrumentation*
Urine / cytology*,  microbiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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