Document Detail

Analysis of urinary organic acids by Plasmaspray liquid chromatography/mass spectrometry.
MedLine Citation:
PMID:  3242679     Owner:  NLM     Status:  MEDLINE    
High-performance liquid chromatography is being used increasingly as a screening method to detect organic acid-urias, an important group of inherited metabolic disorders. Analysis is hampered by lack of a suitable specific detection system. We have carried out preliminary investigations to assess the potential value of liquid chromatography/mass spectrometry with a plasmaspray interface. Spectra of standard acids yielded intense [M-H]- ions with little fragmentation. Organic acids could not be identified in urine samples from healthy neonates because of poor sensitivity. However, urine from a baby with the inherited disorder methylmalonic aciduria showed a distinct peak of methylmalonic acid, easily identified owing to its high sample concentration.
G A Mills; V Walker; M R Clench; V C Parr
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biomedical & environmental mass spectrometry     Volume:  16     ISSN:  0887-6134     ISO Abbreviation:  Biomed. Environ. Mass Spectrom.     Publication Date:  1988 Oct 
Date Detail:
Created Date:  1989-05-26     Completed Date:  1989-05-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8603224     Medline TA:  Biomed Environ Mass Spectrom     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  259-61     Citation Subset:  IM    
Department of Chemical Pathology, University of Southampton, Southampton General Hospital, UK.
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MeSH Terms
Acidosis / urine
Acids / urine*
Chromatography, High Pressure Liquid
Infant, Newborn
Infant, Newborn, Diseases / urine
Mass Spectrometry
Methylmalonic Acid / urine
Spectrophotometry, Ultraviolet
Reg. No./Substance:
0/Acids; 516-05-2/Methylmalonic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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