Document Detail

Analysis of urinary biomarkers for exposure to alkyl benzenes by isotope dilution gas chromatography-mass spectrometry.
MedLine Citation:
PMID:  16405184     Owner:  NLM     Status:  MEDLINE    
A validated GC-MS method for the analysis of urinary metabolites of alkyl benzenes is reported. Metabolites for exposure to toluene, xylene and ethylbenzene were analyzed simultaneously using stable isotope substituted internal standards. The method entailed acidic deconjugation of urine samples followed by extractive alkylation with pentafluorobenzyl bromide as alkylating agent. The resulting pentafluorobenzyl derivatives of ortho-, meta-, para-cresol, mandelic acid (MA), hippuric acid (HA) and ortho-, meta-, para-methylhippuric acid (MHA) were then quantified by SIM. Optimized reaction conditions for the extractive alkylation step are reported. The derivatives were found to be sufficiently stable for overnight batch analysis. The LODs were below 0.1 micromol/L for the cresols and below 1 micromol/L for MA and the HAs. Within-batch precision for o-MHA was 7%, for m-MHA 5%, for p-MHA 5.2% and below 5% for the rest of the analytes.
Adriaan A S Marais; Johannes B Laurens
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of separation science     Volume:  28     ISSN:  1615-9306     ISO Abbreviation:  J Sep Sci     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2006-01-12     Completed Date:  2006-05-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101088554     Medline TA:  J Sep Sci     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2526-33     Citation Subset:  IM    
Department of Chemical Pathology, University of Pretoria, Pretoria, South Africa.
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MeSH Terms
Benzene / analysis*,  toxicity*
Biological Markers
Chemistry / methods*
Gas Chromatography-Mass Spectrometry / instrumentation*,  methods*
Hippurates / analysis
Mandelic Acids / analysis
Models, Chemical
Reproducibility of Results
Sensitivity and Specificity
Time Factors
Urinalysis / methods*
Reg. No./Substance:
0/Biological Markers; 0/Hippurates; 0/Mandelic Acids; 27115-50-0/4-methylhippuric acid; 495-69-2/hippuric acid; 71-43-2/Benzene; 90-64-2/mandelic acid

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