Document Detail


Analysis of the transcriptional activity of the hut promoter in Bacillus subtilis and identification of a cis-acting regulatory region associated with catabolite repression downstream from the site of transcription.
MedLine Citation:
PMID:  1360137     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Levels of transcripts initiated at a hut promoter in Bacillus subtilis were analysed. The addition of histidine to the culture medium increased the level of the transcript sixfold. In the presence of histidine and glucose together, the level of the transcript was reduced to the level in the absence of induction. Furthermore, addition of a mixture of 16 amino acids to cultures of induced cells and of catabolite-repressed cells decreased levels of the transcript 16-fold and 2.6-fold, respectively. Thus, it appears that at least three regulatory mechanisms associated with induction, catabolite repression, and amino acid repression, control the transcriptional activity of the hut promoter. Expression of the hut promoter-lacZ fusions that contained various regions of the hutP gene and deletion analysis of the hutP region revealed a cis-acting sequence associated with catabolite repression that was located between positions +204 and +231 or around position +203.
Authors:
M Oda; T Katagai; D Tomura; H Shoun; T Hoshino; K Furukawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular microbiology     Volume:  6     ISSN:  0950-382X     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  1992 Sep 
Date Detail:
Created Date:  1992-12-24     Completed Date:  1992-12-24     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2573-82     Citation Subset:  IM    
Affiliation:
Fermentation Research Institute, Agency of Industrial Science and Technology, MITI, Ibaraki, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / pharmacology
Bacillus subtilis / genetics*
Bacterial Proteins / genetics,  metabolism
Base Sequence
Gene Expression Regulation, Bacterial* / drug effects
Genes, Bacterial*
Glucose / pharmacology
Histidine / metabolism*,  pharmacology
Histidine Ammonia-Lyase / genetics,  metabolism
Hydrolases / genetics,  metabolism
Molecular Sequence Data
Operon*
Promoter Regions, Genetic*
Recombinant Fusion Proteins / biosynthesis,  genetics
Regulatory Sequences, Nucleic Acid
Transcription, Genetic* / drug effects
Urocanate Hydratase / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Bacterial Proteins; 0/Recombinant Fusion Proteins; 50-99-7/Glucose; 71-00-1/Histidine; EC 3.-/Hydrolases; EC 3.5.3.8/EE57C protein, Bacillus subtilis; EC 4.2.1.49/Urocanate Hydratase; EC 4.3.1.3/Histidine Ammonia-Lyase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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