Document Detail


Analysis of thermal melting curves.
MedLine Citation:
PMID:  15025917     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
T(m) is defined as Temperature of melting or, more accurately, as temperature of midtransition. This term is often used for nucleic acids (DNA and RNA, oligonucleotides and polynucleotides). A thermal denaturation experiment determines the stability of the secondary structure of a DNA or RNA and aids in the choice of the sequences for antisense oligomers or PCR primers. Beyond a simple numerical value (the T(m)), a thermal denaturation experiment, in which the folded fraction of a structure is plotted vs. temperature, yields important thermodynamic information. We present the classic problems encountered during these experiments and try to demonstrate that a number of useful pieces of information can be extracted from these experimental curves.
Authors:
Jean-Louis Mergny; Laurent Lacroix
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Oligonucleotides     Volume:  13     ISSN:  1545-4576     ISO Abbreviation:  Oligonucleotides     Publication Date:  2003  
Date Detail:
Created Date:  2004-03-17     Completed Date:  2004-09-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101188415     Medline TA:  Oligonucleotides     Country:  United States    
Other Details:
Languages:  eng     Pagination:  515-37     Citation Subset:  IM    
Affiliation:
Laboratoire de Biophysique, INSERM UR565, CNRS UMR 5153, Muséum National d'Histoire Naturelle, 75231 Paris, France. mergny@vnumail.com
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MeSH Terms
Descriptor/Qualifier:
Kinetics
Nucleic Acid Denaturation
Oligonucleotides / chemistry*
Spectrophotometry, Ultraviolet
Thermodynamics
Transition Temperature*
Chemical
Reg. No./Substance:
0/Oligonucleotides

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