Document Detail


Analysis of temporal patterns of GPCR-β-arrestin interactions using split luciferase-fragment complementation.
MedLine Citation:
PMID:  23302795     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We developed bioluminescence probes to detect quantitative interaction of GPCRs with arrestin isoforms β-arrestin1 and β-arrestin2 based on split luciferase complementation. Time-dependent GPCR-β-arrestin interactions showed two-types of remarkable variations that were consistent with a classification of GPCR classes. Positive charge residues in serine clusters located at the C-terminal region of GPCRs were necessary for binding to β-arrestin. This quantitative method enables elucidation of the mechanisms of different classes of GPCRs that regulate β-arrestin isoforms.
Authors:
Mitsuru Hattori; Miho Tanaka; Hideo Takakura; Kiyono Aoki; Kenji Miura; Tomohiro Anzai; Takeaki Ozawa
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-10
Journal Detail:
Title:  Molecular bioSystems     Volume:  -     ISSN:  1742-2051     ISO Abbreviation:  Mol Biosyst     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101251620     Medline TA:  Mol Biosyst     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Chemistry, School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. ozawa@chem.s.u-tokyo.ac.jp.
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